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LightMix® Modular Influenza A H1 (H1N1 sw)

Kit with reagents for 96 PCR reactions 20 μl for detection of Inf A H1 RNA [lyophilized]
TIB MOLBIOL order number: 50-0100-96

For in vitro diagnostic use.

Product No. Pack Size
07792204001 96 reactions

To order these products, please contact your Roche order management team.

Influenza A is a negative strand ssRNA Orthomyxo Virus. Infection affects the upper respiratory tract, causing a wide spectrum of symptoms with cough, high fever, and pneumonia, and can be fatal. RT-PCR based detection of Influenza A is based on the conserved matrix protein gene (MP). There are many subtypes of the virus, specified by their Hemagglutinin (H or HA) and Neuraminidase (N or NA) genes. This kit allows to detect the H1 gene found in the H1N1 'swine flu' virus circulation since 2009, which is associcated with more severe case in particular in young individuals.
 

Contents

1 Vial orange cap 96 reactions InfA H1 (lyophilized)
   99.8% oligonucleotides (<0.01 pg)
   0.1% CAS 77-86-1 Tris (hydroxymethyl) aminomethane
   0.1% CAS 60-00-4 Ethylenediamine tetraacetic acid (EDTA)
1 Vial black cap Positive Control, Cp-value ~ 30
 

Additional Reagents Required
LightCycler® Multiplex RNA Virus Master

For use with Roche '480' instruments. Refer to 'Instructions For Use Respiratory Virus Panel I'

Sample Material
Typical clinical samples are extracted from nasopharyngeal swabs, throat swabs, tracheal aspirates, sputum, bronchial wash, or bronchoalveolar lavage.

Target Information and Specification
A 82 bp long fragment from the viral hemagglutinin protein gene is amplified with specific primers and detected with a Cyan500 labeled hydrolysis probe. This assay is developed for use in multiplex PCR and can be run with a control reaction alone or combined with other kits as specified in the Instructions For Use Respiratory Virus Panel I.