LightMix® Modular Influenza B
Kit with reagents for 96 PCR reactions 20 μl for detection of Inf B genomic RNA [lyophilized]
TIB MOLBIOL order number: 58-0102-96
For in vitro diagnostic use.
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Influenza B Virus, a positive strand RNA virus, causes respiratory Influenza primarily in humans with several symptoms like a dry, hacking cough, sore throat, headache and limb pains. Nasal discharge and sneezing are common. The virus is less variable than Inf A and the a large fraction of the population is immunized thus epidemics are less likely than for Influenza A.
1 Vial red cap 96 reactions InfA (lyophilized)
99.8% oligonucleotides (<0.01pg)
0.1% CAS 77-86-1 Tris (hydroxymethyl) aminomethane
0.1% CAS 60-00-4 Ethylenediamine tetraacetic acid (EDTA)1
1 Vial black cap Positive Control, Cp-value ~ 30
Additional Reagents Required
LightCycler® Multiplex RNA Virus Master
For use with Roche '480' instruments. Refer to 'Instructions For Use Respiratory Virus Panel I'
Typical clinical samples are extracted from nasopharyngeal swabs, throat swabs, tracheal aspirates, sputum, bronchial wash, or bronchoalveolar lavage.
Target Information and Specification
A 95 bp long fragment from the viral matrix protein gene is amplified with specific primers and detected with a R6G labeled hydrolysis probe (580 channel). This assay is developed for use in multiplex PCR and can be run with a control reaction alone or combined with other kits as specified in the Instructions For Use Respiratory Virus Panel I. This assay detects 10 genome equivalent copies or less per reaction (in vitro transcribed RNA).