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LightMix® Modular KPC Carbapenemase

Kit with reagents for 96 PCR reactions 20 μl for detection of KPC [lyophilized]
TIB MOLBIOL order number: 61-0626-96

For life science research only. Not for use in diagnostic procedures. For in vitro use only.

Product No. Pack Size
07042272001 96 reactions

To order these products, please contact your Roche order management team.

Antibiotics containing a ß-lactam ring like penicillins and cephamycins are commonly used for treatment of Gram-positive as well as some Gram-negative bacteria. Beta-lactamases cleave the β-lactam ring. Carbapenemases, member of the Extended-spectrum ß-lactamases (ESBL) act also on carbapenems. Carbapenem-Resistant Enterobacteriaceae (CRE) are a growing threat for hospital acquired infections.

The plasmid encoded Class A Klebsiella pneumoniae carbapenemase (KPC) is currently the most common carbapenemase. First detected 1996 in North Carolina it has since then spread worldwide. KPC is found predominantly in Klebsiella and only some reports for Enterobacter and Salmonella exist.
 

Contents

1 Vial purple cap 96 reactions KPC (lyophilized)
1 Vial black cap Positive Control (32,000 copies, lyophilized)

Additional Reagents required

LightCycler® Multiplex DNA Master
or LightCycler® 480 Probes Master

A 122 bp long fragment from the blaKPC gene (types 1-11) is amplified with specific primers and detected with a LC610 labeled hydrolysis probe. In a German study including 60,000 samples the prevalence was 0.05%; sensitivity and specificity were 100%, NPV was 100%, and the PPV was 79.1%. Negative results can be considered as true.

Specification
This assay detects 10 genome equivalent copies or less per reaction (plasmid DNA dilution).

Sample Material and Extraction
Typical samples are from feces, rectal swabs or bacterial culture. See ModularDx Extraction Protocols.