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LightMix® Modular Legionella pneumophila

Kit with reagents for 96 PCR reactions 20 μl for detection of Legionella pn. [lyophilized]
TIB MOLBIOL order number: 50-0683-96

For life science research only. Not for use in diagnostic procedures. For in vitro use only.

Product No. Pack Size
07730586001 96 reactions

To order these products, please contact your Roche order management team.

Legionella pneumophila from the family Legionellaceae is responsible most of the human infections. Many Legionella infections stay subclinical. Pneumonia is the most frequent clinical manifestation The onset is usually abrupt, with high fever, malaise, myalgias, headache, and a non productive cough.
The region of the MIP gene targeted by this kit is specific for L. pneumophila; this kit has been tested to show no cross reactivity with L. longbeachae. Option: The ModularDx kit 64-0681-96 (07 730 608 001) Legionella spp/pn detects all members of the genus Legionella.
 

Contents

1 Vial orange cap 96 reactions Legionella pn. (lyophilized)
1 Vial black cap Positive Control (32,000 copies, lyophilized)

Additional Reagents required

LightCycler® Multiplex DNA Master
or LightCycler® 480 Probes Master

A 148 bp long fragment from the MIP gene of Legionella pn is amplified with specific primers and detected with a Cyan500 labelled hydrolysis probe.

Specification
This assay detects 10 target copies or less per reaction (plasmid DNA dilution).

Sample Material and Extraction
Typical samples are from bronchoalveolare lavage or sputum. See ModularDx Extraction Protocols.