LightMix® Modular MERS-CoV Orf1a
Kit with reagents for 96 PCR reactions 20 μl for detection of MERS Corona virus [lyophilized]
TIB MOLBIOL order number: 53-0635-96
For life science research only. Not for use in diagnostic procedures. For in vitro use only.
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To order these products, please contact your Roche order management team.
Middle East respiratory syndrome coronavirus (MERS-CoV) has been first reported 2012 in Saudi Arabia with currenly (2014-14) more than 200 cases and 89 deaths, most of them in Arabian countries. Positive laboratory results obtained with the upE gene specific test (Cat. No. 06 950 302 001) should be confirmed with a test for the orf1 gene (this kit).
1 Vial yellow cap 96 reactions orf1a (lyophilized)
1 Vial black cap Positive Control (32,000 copies)
Additional Reagents required
LightCycler® Multiplex RNA Virus Master
A 84 bp long fragment upstream of the Orf1a gene is amplified with specific primers and detected with a FAM labeled hydrolysis probe (530 channel).
This assay detects 10 genome equivalent copies or less per reaction.
Sample Material and Extraction
Typical clinical samples are nasopharyngeal swabs, tracheal aspirates or bronchoalveolar lavage. See ModularDx Document Extraction Protocols.