LightMix® Modular MERS-CoV upE
Kit with reagents for 96 PCR reactions 20 μl for detection of MERS Corona virus [lyophilized]
TIB MOLBIOL order number: 53-0624-96
For life science research use only. Not for use in diagnostic procedures. For in vitro use only
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To order these products, please contact your Roche order management team.
Middle East respiratory syndrome coronavirus (MERS-CoV) has been first reported 2012 in Saudi Arabia with currenly (2014-09) more than 700 cases and 302 deaths, most of them in Arabian countries. Positive laboratory results should be confirmed with the MERS specific test targeting the orf1 gene (Cat. No. 07 088 833 001)
1 Vial yellow cap 96 reactions upE (lyophilized)
1 Vial black cap Positive Control (32,000 copies)
Additional Reagents required
LightCycler® Multiplex RNA Virus Master
A 93 bp long fragment upstream of the E gene is amplified with specific primers and detected with a FAM labeled hydrolysis probe (530 channel).
1 -This kit can be applied with the Multiplex RNA master on LightCycler® 2.0 instruments.
2 -This kit can be combined with the Roche Diagnostics Realtime Ready RNA Virus master.
3 -The annealing temperature can be increased up to 64°C (instructions for 1-3 not included).
This assay detects 10 genome equivalent copies or less per reaction (in vitro transcribed RNA).
Sample Material and Extraction
Typical clinical samples are nasopharyngeal or oropharyngeal swabs, lower respiratory tract tracheal aspirates or bronchoalveolar lavage, serum, and possibly also fecal samples. See ModularDx Document Extraction Protocols.