LightMix® Modular MS2 RNA Extraction Control

Kit with reagents for 96 PCR reactions 20 μl for detection of phage MS RNA [lyophilized]
TIB MOLBIOL order number: 66-0908-96

For life science research only. Not for use in diagnostic procedures. For in vitro use only. 

Product No. Pack Size
07094221001 96 reactions

To order these products, please contact your Roche order management team.

PCR analysis of biological samples may occasionally result difficult due to problems related to the extraction process. In particular, false negative results in pathogen testing can be due to the presence of inhibitors, degraded target material or an unsuccessful extraction of nucleic acids. The presence of amplifiable nucleic acids can be verified by running an extraction control PCR.

This product is intended to be used as extraction control and as reference gene for RNA-based assays.
The target is phage MS RNA.
 

Contents

1 Vial Vial green cap 96 reactions MS2 (lyophilized)

Additional Reagents required

  • Realtime Ready RNA Virus Master
  • MS RNA

A 143 bp long fragment from the 5-UTR and 5'-region of the assembly protein gene of phage MS2 is amplified with specific primers and detected with a hydrolysis probe with Cy5 label and dark quencher.

Specification
This kit detects 0.01 ng or less of target RNA. The linear measuring range is 10 - 106 DNA copies (plasmid).

Sample Material and Extraction
Depends on the analytical PCR. See ModularDx Document Extraction Protocols.