LightMix® Modular MSTN Extraction Control (580)
Kit with reagents for 96 PCR reactions 20 μl for detection of MSTN [lyophilized]
TIB MOLBIOL order number: 58-0905-96
For life science research only. Not for use in diagnostic procedures. For in vitro use only.
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To order these products, please contact your Roche order management team.
This product is intended to be used as extraction control and as reference gene for DNA or RNA-based assays. The targeted region of the Myostatin (MSTN) gene is highly conserved in chordata.
For use with capillary LightCycler instruments include a seek dye to ensure to detect the capillaries.
1 Vial red cap 96 reactions MSTN (lyophilized)
1 Vial seek dye ROX (330 μl)
A 89 bp long fragment from the myostatin gene or transcript is amplified with specific primers and detected with a hydrolysis probe with a R6G labeled hydrolysis probe. Starting with lot 3407 this kit has been improved by adding one primer and one probe to detect a wider variety of species.
These reagents detect 0.01 ng or less of vertebrate DNA. The linear measuring range is 0.01 to 500 ng DNA.
The product has been confirmed to work with samples from human (Homo sapiens), porc (Sus suis), beef (bos bovis), dromedary (Camelus dromedarius), chicken (Gallus gallus), turkey (Meleagris gallopavo) ostrich (Struthio camelus), mallard (Anas platyrhynchos), pigeon (Columba livia), quail (Coturnix coturnix) guinea fowl (Numida meleagris), duck muscovy (Cairina moschata), and goose (Anser ansermallard).
Sample Material and Extraction
Depends on the analytical PCR (DNA or RNA). See ModularDx Document Extraction Protocols.
Additional reagents required
LightCycler® Multiplex RNA Virus Master
LightCycler® Multiplex DNA Master
LightCycler® 480 Probes Master