LightMix® Modular MSTN Extraction Control (660)

Kit with reagents for 96 PCR reactions 20 μl for detection of MSTN [lyophilized]
TIB MOLBIOL order number: 66-0905-96

For life science research only. Not for use in diagnostic procedures. For in vitro use only

Product No. Pack Size
07225253001 96 reactions

To order these products, please contact your Roche order management team.

Keywords: Control Kits

PCR analysis of biological samples may occasionally result difficult due to problems related to the extraction process. In particular, false negative results in pathogen testing can be due to the presence of inhibitors, degraded target material or an unsuccessful extraction of nucleic acids. The presence of amplifiable nucleic acids can be verified by running an extraction control PCR.

This product is intended to be used as extraction control and as reference gene for DNA or RNA-based assays. The targeted region of the Myostatin (MSTN) gene is highly conserved in chordata.
 

Contents

Vial green cap 96 reactions MSTN (lyophilized)

Additional Reagents required

  • LightCycler® Multiplex RNA Virus Master
  • LightCycler® Multiplex DNA Master (if only DNA targets tested)
    or
  • LightCycler® 480 Probes Master

A 89 bp long fragment from the myostatin gene or transcript is amplified with specific primers and detected with a LC670 labeled hydrolysis probe. Starting with lot 3370 this kit has been improved by adding one primer and one probe to detect a wider variety of species.

Specification
These reagents detect 0.01 ng or less of vertebrate DNA. The linear measuring range is 0.01 to 500 ng DNA. The product has been confirmed to work with samples from human (Homo sapiens), porc (Sus suis), beef (bos bovis), dromedary (Camelus dromedarius), chicken (Gallus gallus), turkey (Meleagris gallopavo) ostrich (Struthio camelus), mallard (Anas platyrhynchos), pigeon (Columba livia), quail (Coturnix coturnix) guinea fowl (Numida meleagris), duck muscovy (Cairina moschata), and goose (Anser ansermallard).

Sample Material and Extraction
Depends on the analytical PCR. See ModularDx Document Extraction Protocols.