LightMix® Modular Mycoplasma pneumoniae
Kit with reagents for 96 PCR reactions 20 μl for detection of Mycoplasma pn. [lyophilized]
TIB MOLBIOL order number: 53-0680-96
For life science research only. Not for use in diagnostic procedures. For in vitro use only.
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To order these products, please contact your Roche order management team.
Mycoplasma pneumoniae is the most frequent bacterial respiratory pathogen and belongs together with Chlamydia pn. and Legionella to the three common agents causing 'Atypical Pneumonia'.
M. pneumoniae causes tracheobronchitis and pneumonia with symptoms of nonproductive cough, sore throat, and middle-ear involvement.
1 Vial yellow cap 96 reactions Mycoplasma pn.(lyophilized)
1 Vial black cap Positive Control (32,000 copies, lyophilized)
Additional Reagents required
LightCycler® Multiplex DNA Master
or LightCycler® 480 Probes Master
A 89 bp fragment of the repMp1 gene of Mycoplasma pneumonia is amplified with specific primers and detected with a FAM labeled hydrolysis probe.
This assay detects 10 genome equivalent copies or less per reaction (plasmid DNA dilution).
Sample Material and Extraction
Typical samples are from bronchoalveolare lavage or sputum. See ModularDx Extraction Protocols.