LightMix® Modular Neisseria meningitidis
Kit with reagents for 96 PCR reactions 20 μl for detection of N. meningitidis [lyophilized]
TIB MOLBIOL order number: 61-0644-96
For life science research only. Not for use in diagnostic procedures. For in vitro use only.
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To order these products, please contact your Roche order management team.
Bacterial meningitis in adults is caused mainly by Neisseria meningitidis or Streptococcus pneumoniae. Children might have Haemophilus influenzae while newborn can be also affected by Streptococcus agalactiae and Escherichia coli. Listeria monocytogenes is known to cause meningitis in elderly persons and newborns.
1 Vial purple cap 96 reactions N. meningitidis (lyophilized)
1 Vial black cap Positive Control (32,000 copies, lyophilized)
Additional Reagents required
LightCycler® Multiplex DNA Master
LightCycler® 480 Probes Master
A 81 bp long fragment from the capsular transport protein (ctrA) gene is amplified with specific primers and detected with a LC610 labeled hydrolysis probe.
This assay detects 10 target copies or less per reaction (plasmid DNA dilution).
Sample Material and Extraction
Typical sample type is cerebrospinal fluid (CSF). See ModularDx Document Extraction Protocols.