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LightMix® Modular Norovirus GG1 (500)

Kit with reagents for 96 PCR reactions 20 μl for detection of Norovirus GG1 RNA [lyophilized]
TIB MOLBIOL order number: 50-0652-96

For in vitro diagnostic use.

Product No. Pack Size
07792239001 96 reactions

To order these products, please contact your Roche order management team.

Infectious diarrhea can be caused by viruses, bacteria or parasites. About 50% of all gastroenteritis is caused by Norovirus (NoV), a positive strand RNA virus with a high genomic variability. NoV are classified into 5 genogroups (GG), from which GG2 and to less extend GG1 (or GG4) infect humans.
 

Contents

1 Vial orange cap 96 reactions NoV GG1 (lyophilized)
   99.8% oligonucleotides (<0.01 pg)
   0.1% CAS 77-86-1 Tris (hydroxymethyl) aminomethane
   0.1% CAS 60-00-4 Ethylenediamine tetraacetic acid (EDTA)
1 Vial black cap Positive Control, Cp-value ~ 30
 

Additional Reagents Required
LightCycler® Multiplex RNA Virus Master

For use with Roche '480' instruments. Refer to 'Instructions For Use Gastro Virus'

Sample Material
Typical clinical samples are extracted from feces or rectal swabs.

Target Information and Specification
A 70 bp long fragment from the NoV junction sequence between the orf1 and orf2 genes is amplified with specific primers and detected with a Cyan500 labeled hydrolysis probe (500 channel).
This assay is developed for use in multiplex PCR and can be run with a control reaction alone or combined with other kits as specified in the Instructions For Use Gastro Virus'.