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LightMix® Modular Parechovirus (hPeV)

Kit with reagents for 96 PCR reactions 20 μl for detection of hPev RNA [lyophilized]
TIB MOLBIOL order number: 53-0126-96

For life science research only. Not for use in diagnostic procedures. For in vitro use only.

Product No. Pack Size
07730438001 96 reactions

To order these products, please contact your Roche order management team.

Parechovirus (PeV) splits in two species A and B belonging to the familiy of Picornaviridae with six human pathogen viruses hPeV1 to hPEV6 (formerly accounted as echovirus). They cause mild, gastrointestinal or respiratory illness, but have been implicated in cases of myocarditis and encephalitis. Most humans are infected within an age of less than five years.
 

Contents

1 Vialyellow cap 96 reactions hPeV (lyophilized)
1 Vial black cap Positive Control, Cp-value ~ 31

Additional Reagents required
LightCycler® Multiplex RNA Virus Master

A 78 bp long fragments from the 5-UTR region is amplified with specific primers and detected with a FAM labeled hydrolysis probe (530 channel).

Specification
This assay detects 10 genome equivalent copies or less per reaction (in vitro transcribed RNA).

Sample Material and Extraction
Typical clinical samples are nasopharyngeal swabs or from feces. See ModularDx Document Extraction Protocols.