LightMix® Modular PhHV Internal Control
Kit with reagents for 96 PCR reactions 20 μl for detection of PhHV [lyophilized]
TIB MOLBIOL order number: 66-0625-96
For life science research only. Not for use in diagnostic procedures. For in vitro use only.
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This product is intended to be used as Internal Control to ensure for parameter-negative samples that PCR is working and no inhibitors are present. The amount of target included in the reagent is adjusted to yield a Cp value in the range of 29-30.
The kit 66-0901-96 Roche Cat. No. 07 093 802 001 contains separate target to be used as spiked Extraction Control (EC).
1 Vial green cap 96 reactions IC (lyophilized)
Additional Reagents required
- LightCycler® Multiplex RNA Virus Master
- LightCycler® Multiplex DNA Master (if only DNA targets tested)
- LightCycler® 480 Probes Master
A 85 bp long fragment from the cloned Phocine herpesvirus (PhHV) sequence target is amplified with specific primers and detected with a LC670 labeled hydrolysis probe.
This assay shall yield a Cp value of about 30 for analytical-PCR negative samples in multiplex PCR.
Sample Material and Extraction
Not applicable - depends on the analytical PCR.