LightMix® Modular PhHV spiked Extraction Control (610)
Kit with reagents for 96 PCR reactions 20 μl for detection of PhHV [lyophilized]
TIB MOLBIOL order number: 61-0901-96
For life science research only. Not for use in diagnostic procedures. For in vitro use only.
|Product No.||Pack Size|
To order these products, please contact your Roche order management team.
This product is intended to be used as spiked extraction control and internal control to ensure for parameter-negative samples that extraction and PCR have been working and no inhibitors are present. The amount of target spiked to the samples has to be adjusted to yield a Cp value in the range of 30.
1 Vial purple cap 96 reactions PhHV (lyophilized)
1 Vial black cap containing the Extraction Control Target DNA
Additional Reagents required
- LightCycler® Multiplex DNA Master
- LightCycler® Multiplex RNA Virus Master
- LightCycler® 480 Probes Master
A 85 bp long fragment from the Phocine herpesvirus (PhHV) sequence target is amplified with specific primers and detected with a LC610 labeled hydrolysis probe.
This assay shall yield a Cp value of about 30 for analytical-PCR negative samples in multiplex PCR.
Sample Material and Extraction
Depends on the analytical PCR. See ModularDx Document Extraction Protocols.