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LightMix® Modular PhHV spiked Extraction Control (610)

Kit with reagents for 96 PCR reactions 20 μl for detection of PhHV [lyophilized]
TIB MOLBIOL order number: 61-0901-96

For life science research only. Not for use in diagnostic procedures. For in vitro use only.

Product No. Pack Size
07654286001 96 reactions

To order these products, please contact your Roche order management team.

This product is intended to be used as spiked extraction control and internal control to ensure for parameter-negative samples that extraction and PCR have been working and no inhibitors are present. The amount of target spiked to the samples has to be adjusted to yield a Cp value in the range of 30.
 

Contents

1 Vial purple cap 96 reactions PhHV (lyophilized)
1 Vial black cap containing the Extraction Control Target DNA

Additional Reagents required

  • LightCycler® Multiplex DNA Master
  • LightCycler® Multiplex RNA Virus Master
    or
  • LightCycler® 480 Probes Master

A 85 bp long fragment from the Phocine herpesvirus (PhHV) sequence target is amplified with specific primers and detected with a LC610 labeled hydrolysis probe.

Specification
This assay shall yield a Cp value of about 30 for analytical-PCR negative samples in multiplex PCR.

Sample Material and Extraction
Depends on the analytical PCR. See ModularDx Document Extraction Protocols.