LightMix® Modular Pneumocystis jirovecii (PCP)
Kit with reagents for 96 PCR reactions 20 μl for detection of Pneumocystis jirovecii [lyophilized]
TIB MOLBIOL order number: 50-0689-96
For life science research only. Not for use in diagnostic procedures. For in vitro use only.
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To order these products, please contact your Roche order management team.
Pneumocystis jiroveci is an ascomycetous fungus that causes in patients with impaired immunity opportunistic pneumonia (PCP). Symptoms range from dyspnoea and dry cough to acute respiratory failure. Increasing evidence of human to human transmission supports an early diagnosis of the disease to ensure treatment with appropriate antibiotics. P. jirovecii can be not cultured. Classical diagnosis is based on the microscopic examination of respiratory specimens. PCR diagnosis is based on the detection of the rRNA genes, the heat-shock protein 70B/SSB1 gene, the DHFR gene or the multicopy surface glycoprotein (MSG) gene.
1 Vial orange 96 reactions Pneumocystis jirovecii (lyophilized)
1 Vial black cap Positive Control (32,000 copies, lyophilized)
Additional Reagents required
LightCycler® Multiplex DNA Master
or LightCycler® 480 Probes Master
A 216-240 bp long fragment from the surface glycoprotein (MSG) gene is amplified with specific primers and detected with a Cyan500 labeled hydrolysis probe.
This assay detects 10 genome equivalent copies or less per reaction (plasmid DNA dilution).
Sample Material and Extraction
Typical clinical samples are sputum or bronchoalveolare lavage. See ModularDx Extraction Protocols.