LightMix® Modular RNase P RNA
Kit with reagents for 96 PCR reactions 20 μl for detection of RNase P RNA [lyophilized]
TIB MOLBIOL order number: 58-0911-96
For life science research only. Not for use in diagnostic procedures. For in vitro use only.
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To order these products, please contact your Roche order management team.
PCR analysis of biological samples may fail due to problems related to the nucleic acid extraction. In particular, false negative results in pathogen testing can be due to the presence of inhibitors, degraded target material or an unsuccessful extraction of nucleic acids. The presence of amplifiable nucleic acids can be verified by running an extraction control PCR reaction.
This product is intended to be used as extraction control for RNA-based PCR assays containing human mRNA. The preferred sample type are for example swabs, containing low amounts of human material; samples extracted for example from blood may contain too much target thus the RNase P assay may outcompete the analytical assay. The detection limit of the analytical assay has to be tested in combination with the control assay. This assay is derived from a published CDC assay, with one primer shifted to be transcript-specific - this kit will different from the CDC assay not work with genomic DNA.
1 Vial red cap 96 reactions RNase P RNA (lyophilized)
Additional Reagents required
LightCycler® Multiplex RNA Virus Master
A 89 bp long fragment from the human RNase P mRNA transcript is amplified with specific primers and detected with a R6G labeled hydrolysis probe (580 channel).
These reagents detect 1,000 copies or less transcript.
Sample Material and Extraction
Depends on the analytical PCR. See ModularDx Document Extraction Protocols.