LightMix® Modular Rotavirus A
Kit with reagents for 96 PCR reactions 20 μl for detection of Rotavirus A RNA [lyophilized]
TIB MOLBIOL order number: 58-0654-96
For life science research only. Not for use in diagnostic procedures. For in vitro use only.
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To order these products, please contact your Roche order management team.
Rotavirus infections are the most frequent cause of severe diarrhoea in children; adults are normally immunized from previous infections. The virus is faecal-oral transmitted. Infections are more common in the winter time. The virus has a double stranded RNA genome which is difficult to denature prior PCR amplification. From the five species of Rotavirus A-E most infections are caused by type A.
1 Vial red cap 96 reactions Rotavirus A (lyophilized)
1 Vial black cap Positive Control (RNA), Cp-value ~ 30
Additional Reagents required
LightCycler® Multiplex RNA Virus Master
A 87 bp fragment from the NSP5 gene (segment 11) is amplified with specific primers and detected with specific R6G labeled hydrolysis probes (580 channel).
This assay detects 10 genome equivalent copies or less per reaction (in vitro transcripted RNA).
Sample Material and Extraction
Typical specimen are from feces. See ModularDx Document Extraction Protocols.