LightMix® Modular Streptococcus pneumoniae
Kit with reagents for 96 PCR reactions 20 μl for detection of S. pneumoniae [lyophilized]
TIB MOLBIOL order number: 58-0643-96
For life science research only. Not for use in diagnostic procedures. For in vitro use only.
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To order these products, please contact your Roche order management team.
Bacterial Meningitis in adults is caused mainly by Neisseria meningitidis or Streptococcus pneumoniae, in elderly persons also Listeria monocytogenes. Children might have Haemophilus influenzae while newborn can be also affected by Streptococcus agalactiae and Escherichia coli.
1 Vial red cap 96 reactions S. pneumoniae (lyophilized)
1 Vial black cap Positive Control (32,000 copies, lyophilized)
Additional Reagents required
LightCycler® Multiplex DNA Master
or LightCycler® 480 Probes Master
A 79 bp long fragment from the pneumolysin gene is amplified with specific primers and detected with a R6G labeled hydrolysis probe (580 channel).
This assay detects 10 genome equivalent copies or less per reaction (plasmid DNA dilution).
Sample Material and Extraction
For meningitis testing typical samples are cerebrospinal fluid (CSF), for pneumonia testing from bronchoalveolare lavage or sputum. See ModularDx Document Extraction Protocols.