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LightMix® Modular T. whipplei

Kit with reagents for 96 PCR reactions 20 μl for detection of Tropheryma whipplei [lyophilized]
TIB MOLBIOL order number: 53-0606-96

For life science research only. Not for use in diagnostic procedures. For in vitro use only.

Product No. Pack Size
07094272001 96 reactions

To order these products, please contact your Roche order management team.

Morbus Whipple is a very rare disease caused by Tropheryma whipplei (Incidence less than 1:1000,000). The four classic signs are loss of weight, abdominal pain, diarrhoea and arthralgia. Infectious diarrhea can be caused by several viruses, bacteria or parasites.
 

Contents

1 Vial yellow 96 reactions Tropheryma (lyophilized)
1 Vial black cap Positive Control (32,000 copies, lyophilized)

Additional Reagents required

LightCycler® Multiplex DNA Master
or LightCycler® 480 Probes Master

A 75 bp long fragment from the 'genomic repeated sequence' is amplified with specific primers and detected with a FAM labeled hydrolysis probe.

The assay was compared in a clinical study of 640 diarrhea fecal samples from Africa with a reference assay based on the rpoB gene. The prevalence for T. whipplei was 37%. Sensitivity and specificity were 100%, NPV and PPV were 100%.

Specification
This assay detects 10 genome equivalent copies or less per reaction (plasmid DNA dilution). PCR has been tested to work between 52°C and 64°C annealing temperature.

Sample Material and Extraction
Typical samples are from feces, rectal swabs or bacterial culture. See ModularDx Extraction Protocols.