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MagNA Pure Compact Nucleic Acid Isolation Kit I - Large Volume

Ready-to-use hands-free reagents in prefilled, sealed cartridges for purifying DNA from mammalian cells and total viral nucleic acids from serum and plasma in a final volume of up to one milliliter with the MagNA Pure Compact Instrument.

For general laboratory use.

Product No. Pack Size
03730972001 32 isolations

To order these products, please contact your Roche order management team.

  • Isolate genomic DNA from mammalian whole blood or cultured cells.
  • Isolate viral nucleic acids from serum or plasma. 

Contents

  • Reagent Cartridge, 32 sealed cartridges
  • Tip Tray, 32 disposable Tip Trays
  • MagNA Pure Tubes, 2.0 mL
  • MagNA Pure Elution Tube Caps

For general laboratory use.

The MagNA Pure Compact Nucleic Acid Isolation Kit I – Large Volume is specifically designed to isolate highly purified genomic DNA from mammalian whole blood or cultured cells, and total nucleic acids (e.g., viral DNA and RNA) from mammalian serum or plasma, using the MagNA Pure Compact Instrument. In combination with the MagNA Pure DNA Tissue Lysis Buffer, this kit can be used to isolate DNA from 1 - 10 mg mammalian tissue.

The purified nucleic acids can be used directly in PCR or RT-PCR on the LightCycler® Carousel-Based Systems, the LightCycler® 480 System, or standard thermal block cyclers.

Number of isolations/sample type: 
32 isolations (4 × 8) from

  • 500 or 1,000 μl mammalian whole blood (containing no more than 1 × 104 cells/μl)
  • 500 or 1,000 μl mammalian serum or plasma
  • 100 μl suspension of cultured cells (containing no more than 2 × 106 cells)
  • 200 µl lysate in MagNA Pure DNA Tissue Lysis Buffer (1 – 10 mg mammalian tissue)

MagNA Pure DNA Tissue Lysis Buffer:

  • 1 to 10 mg of mammalian tissue

For details, see - Instructions for Use

To isolate genomic DNA or total nucleic acid from smaller-volume samples (100 to 400 μl), choose the MagNA Pure Compact Nucleic Acid Isolation Kit I 

Product Citations

  • Binnicker, MJ et al. (2007). Detection of Coccidioides Species in Clinical Specimens by Real-Time PCR. J. Clin. Microbiol. 45: 173-178
  • Kirchgesser M et al. (2003). The New MagNA Pure Compact Nucleic Acid Isolation Kits – Fast and Flexible Fully Automated Sample Preparation. Biochemica 4 , 2003: 12 

Principle

The nucleic acid isolation procedure is based on the proven MagNA Pure Magnetic Glass Particle Technology.

The principle steps of a MagNA Pure Compact nucleic acid isolation procedure are:

  • The samples are lysed by incubation with Proteinase K and a special lysis buffer containing a chaotropic salt.
  • Magnetic Glass Particles (MGPs) are added and nucleic acids are immobilized on the MGPs surfaces.
  • Unbound substances (e.g., proteins, cell debris, PCR inhibitors etc) are removed by several washing steps.
  • Purified nucleic acids are eluted from the MGPs.