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Rabbit Anti-Human B7-H3/CD276 Monoclonal Antibody (Clone SP265)

For research use only. Not for use in diagnostics.

Product No. Pack Size
07392435001 0.1 mL
Product No. Pack Size
07392451001 1.0 mL

To order these products, please contact your Roche order management team.

B7-H3 (also known as CD276) is a membrane protein that participates in the regulation of T-cellmediated immune response. B7-H3 can activate both acquired and innate immunity leading to natural killer cell and CD8 T-cell dependent killing of tumor cells. High levels of B7-H3 are seen in antigen presenting cells, some tumor cells, and tumor vasculature. It is the target of anticancer immunotherapy.

Figure above:
Human ovarian adenocarcinoma stained with anti-B7-H3 antibody

Contents

Rabbit monoclonal antibody purified by protein A/G in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.

IHC Performance

 

Human ovarian adenocarcinoma stained with anti-B7-H3 antibody

Human lung squamous cell carcinoma stained with anti-B7-H3 antibody

Human renal cell carcinoma stained with anti-B7-H3 antibody

Human liver hepatoma stained with anti-B7-H3 antibody

Human ovarian adenocarcinoma stained with anti-B7-H3 antibody.

Human lung squamous cell carcinoma stained with anti-B7-H3 antibody.

Human renal cell carcinoma stained with anti-B7-H3 antibody

Human liver hepatoma stained with anti-B7-H3 antibody

Human bladder transitional cell carcinoma stained with anti-B7-H3 antibody

Human cervical squamous cell carcinoma stained with anti-B7-H3 antibody

Human skin squamous cell carcinoma stained with anti-B7-H3 antibody

Human skin melanoma stained with anti-B7-H3 antibody

Human bladder transitional cell carcinoma stained with anti-B7-H3 antibody

Human cervical squamous cell carcinoma stained with anti-B7-H3 antibody

Human skin squamous cell carcinoma stained with anti-B7-H3 antibody

Human skin melanoma stained with anti-B7-H3 antibody

Human Hodgkin's lymphoma stained with anti-B7-H3 antibody

Human prostate adenocarcinoma stained with anti-B7-H3 antibody

Human Hodgkin's lymphoma stained with anti-B7-H3 antibody

Human prostate adenocarcinoma stained with anti-B7-H3 antibody



Flow Cytometric Analysis

 


Flow cytometric analysis of rabbit anti-B7-H3/CD276 (SP265) antibody in PC-3 cells (green) compare to negative control of rabbit IgG (blue)

 

Western Blot Analysis

 


Western Blot analysis of Hs700T cell lysate with B7-H3/CD276 antibody

Product Details:

 

Technology

Rabbit Monoclonal

Clone Name

SP265

Application

IHC-P, WB, FACS

Species Reactivity

 

   Tested

Hu

   Predicted by homology

Ms, Rt

Isotype

Rabbit IgG

Immunogen

Synthetic peptide derived from the C-terminus of human B7-H3/CD276 protein.

Cellular Localization

Membrane

Control Tissue

Ovarian Adenocarcinoma

Regulatory Rating

Research Use Only (RUO)

Entrez

80381

Storage Requirement

2-8°C

Target Molecular Weight

110 kDa

Formulation

Refer to Data Sheet