Rabbit Anti-Human Calpastatin Monoclonal Antibody (Clone SP82)
For research use only. Not for use in diagnostics.
Product No. | Pack Size |
---|---|
06972047001 | 0.1 mL |
Product No. | Pack Size |
---|---|
06972071001 | 1.0 mL |
To order these products, please contact your Roche order management team.
Calpastatin is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. The protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins. The calpain system has been detected in every vertebrate tissue examined, and has been suggested to play a regulatory role in cellular protein metabolism. This regulatory role may have important implications in platelet aggregation and pathologies associated with altered calcium homeostasis and protein metabolism such as ischemic cell injury and degenerative diseases. Inhibitors of calpain have been shown to block dexamethasone and low-level irradiation induced apoptosis in thymocytes suggesting that calpain has a regulatory or mechanistic role in apoptotic cell death. Calpastatin, a specific endogenous inhibitor of calpain, has a predicted molecular weight of ~77kDa and an internal repeat of four homologous domains which allow it to inhibit multiple calpain molecules simultaneously. It functions by binding to calpain when calpain binds calcium. When calpastatin is subjected to proteolytic digestion, fragments of calpastatin as small as 15 kDa still retain inhibitory activity.
Figure above:
Human Breast Carcinoma stained with anti-Calpastatin antibody
Contents
Rabbit monoclonal antibody purified by protein A/G in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.
IHC Performance
Human Breast Carcinoma stained with anti-Calpastatin antibody |
Human Breast Ductal Carcinoma stained with anti-Calpastatin antibody |
Human Breast stained with anti-Calpastatin antibody |
Human Hepatocellular Carcinoma stained with anti-Calpastatin antibody |
Human Bladder stained with anti-Calpastatin antibody |
Human Bladder Transitional Cell Carcinoma stained with anti-Calpastatin antibody |
Human Lung Adenocarcinoma stained with anti-Calpastatin antibody |
Human Melanoma stained with anti-Calpastatin antibody |
Human Prostate Adenocarcinoma stained with anti-Calpastatin antibody |
Human Prostate stained with anti-Calpastatin antibody |
Human Stomach Adenocarcinoma stained with anti-Calpastatin antibody |
Product Details:
Technology |
Rabbit Monoclonal |
Clone Name |
SP82 |
Application |
IHC-P |
Species Reactivity |
|
Tested |
Hu |
Predicted by homology |
Bv, Ck, Pg, Rb, Rt |
Isotype |
Rabbit IgG |
Immunogen |
Synthetic peptide derived from near C-terminus of human calpastatin protein. |
Cellular Localization |
Cytoplasm |
Control Tissue |
Breast Carcinoma |
Regulatory Rating |
Research Use Only (RUO) |
Entrez |
831 |
Storage Requirement |
2-8°C |
Target Molecular Weight |
77 kDa |
Formulation |
Refer to Data Sheet |