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Rabbit Anti-Human CD20 Monoclonal Antibody (Clone SP32)

For research use only. Not for use in diagnostics.

Product No. Pack Size
05493234001 0.1 mL
Product No. Pack Size
05298938001 1.0 mL

To order these products, please contact your Roche order management team.

CD20 is a 33kDa leukocyte surface antigen consisting of four transmembrane regions and cytoplasmic N- and C-termini. CD20 is expressed primarily on B cells but has also been detected on both normal and neoplastic T cells. Activation of CD20 significantly increases DNA synthesis and is thought to involve basic helix-loop-helix leucine zipper transcription factors.

Figure above:
Human B Cell Lymphoma stained with anti-CD20 antibody

Contents

Rabbit monoclonal antibody purified by protein A/G in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.

IHC Performance

 

Human B Cell Lymphoma stained with anti-CD20 antibody

Human B Cell Lymphoma stained with anti-CD20 antibody2

Human Cervical Squamous Cell Carcinoma stained with anti-CD20 antibody

Human Colon stained with anti-CD20 antibody

Human B Cell Lymphoma stained with anti-CD20 antibody

Human B Cell Lymphoma stained with anti-CD20 antibody2

Human Cervical Squamous Cell Carcinoma stained with anti-CD20 antibody

Human Colon stained with anti-CD20 antibody

Human HK Lymphoma stained with anti-CD20 antibody

Human Lung Adenocarcinoma stained with anti-CD20 antibody

Human Reactive Lymph Node stained with anti-CD20 antibody

Human Skin Squamous Cell Carcinoma stained with anti-CD20 antibody

Human HK Lymphoma stained with anti-CD20 antibody

Human Lung Adenocarcinoma stained with anti-CD20 antibody

Human Reactive Lymph Node stained with anti-CD20 antibody

Human Skin Squamous Cell Carcinoma stained with anti-CD20 antibody

Human Spleen stained with anti-CD20 antibody

Human Thymus stained with anti-CD20 antibody

Human Tonsil stained with anti-CD20 antibody

Human Tonsil stained with anti-CD20 antibody

Human Spleen stained with anti-CD20 antibody

Human Thymus stained with anti-CD20 antibody

Human Tonsil stained with anti-CD20 antibody

Human Tonsil stained with anti-CD20 antibody.

 

Flow Cytometric Analysis

 

Flow cytometric analysis of rabbit anti-CD20 (SP32) antibody in Jurkat (green) compare to negative control of rabbit IgG (blue)
Flow cytometric analysis of rabbit anti-CD20 (SP32) antibody in Jurkat (green) compare to negative control of rabbit IgG (blue)



Western Blot Analysis

Western Blot analysis of anti-CD20 antibody
Western Blot analysis of anti-CD20 antibody

Product Details:

 

Technology

Rabbit Monoclonal

Clone Name

SP32

Application

IHC-P, WB, FACS

Species Reactivity

 

   Tested

Hu

   Predicted by homology

Bv, Dg

Isotype

Rabbit IgG

Immunogen

Synthetic peptide from C-terminus of human CD20 protein.

Cellular Localization

Cytoplasm, Membrane

Control Tissue

B Cell Lymphoma, Tonsil

Regulatory Rating

Research Use Only (RUO)

Entrez

931

Storage Requirement

2-8°C

Target Molecular Weight

35 kDa

Formulation

Refer to Data Sheet