Rabbit Anti-Human CD3 Monoclonal Antibody (Clone SP162)
For research use only. Not for use in diagnostics.
Product No. | Pack Size |
---|---|
06975020001 | 0.1 mL |
Product No. | Pack Size |
---|---|
06975046001 | 1.0 mL |
To order these products, please contact your Roche order management team.
This antibody reacts with the intracytoplasmic portion of the CD3 antigen expressed by T cells. It stains human T cells in both the cortex and medulla of the thymus and in peripheral lymphoid tissues. This antibody is suitable for staining normal and neoplastic T cells in formalin-fixed, paraffin-embedded tissues.
Figure above:
Human Tonsil stained with anti-CD3 antibody
Contents
Rabbit monoclonal antibody purified by protein A/G in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.
IHC Performance
Human B-cell lymphoma stained with anti-CD3 antibody |
Human Bladder stained with anti-CD3 antibody |
Human Cervix stained with anti-CD3 antibody |
Human Colon stained with anti-CD3 antibody |
Human Esophagus stained with anti-CD3 antibody |
Human Fallopian Tube stained with anti-CD3 antibody |
Human HK lymphoma stained with anti-CD3 antibody |
Human Liver stained with anti-CD3 antibody |
Human Spleen stained with anti-CD3 antibody |
Human Thymus stained with anti-CD3 antibody |
Human Tonsil stained with anti-CD3 antibody |
Flow Cytometric Analysis
Flow cytometric analysis of rabbit anti-CD3 (SP162) antibody in Jurkat (green) compare to negative control of rabbit IgG (blue)
Western Blot Analysis
Western Blot analysis of Jurkat cell lysate with CD3 antibody
Product Details:
Technology |
Rabbit Monoclonal |
Clone Name |
SP162 |
Application |
IHC-P, WB, FACS |
Species Reactivity |
|
Tested |
Hu |
Predicted by homology |
Bv, Ck, Dg, Ms, Pg, Rb, Rt |
Isotype |
Rabbit IgG |
Immunogen |
Synthetic peptide corresponding to internal region of the epsilon chain of human CD3 protein. |
Cellular Localization |
Membrane |
Control Tissue |
Tonsil |
Regulatory Rating |
Research Use Only (RUO) |
Entrez |
916 |
Storage Requirement |
2-8°C |
Target Molecular Weight |
20 kDa |
Formulation |
Refer to Data Sheet |