Rabbit Anti-Human CD3 Monoclonal Antibody (Clone SP162)

For research use only. Not for use in diagnostics.

Product No.	Pack Size
06975020001	0.1 mL

Product No.	Pack Size
06975046001	1.0 mL

To order these products, please contact your Roche order management team.

Keywords: Antibodies, Rabbit Monoclonal

This antibody reacts with the intracytoplasmic portion of the CD3 antigen expressed by T cells. It stains human T cells in both the cortex and medulla of the thymus and in peripheral lymphoid tissues. This antibody is suitable for staining normal and neoplastic T cells in formalin-fixed, paraffin-embedded tissues.

Figure above:
Human Tonsil stained with anti-CD3 antibody

Contents

Rabbit monoclonal antibody purified by protein A/G in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.

IHC Performance


Human B-cell lymphoma stained with anti-CD3 antibody	Human Bladder stained with anti-CD3 antibody	Human Cervix stained with anti-CD3 antibody	Human Colon stained with anti-CD3 antibody

Human Esophagus stained with anti-CD3 antibody	Human Fallopian Tube stained with anti-CD3 antibody	Human HK lymphoma stained with anti-CD3 antibody	Human Liver stained with anti-CD3 antibody

Human Spleen stained with anti-CD3 antibody	Human Thymus stained with anti-CD3 antibody	Human Tonsil stained with anti-CD3 antibody

Flow Cytometric Analysis

Flow cytometric analysis of rabbit anti-CD3 (SP162) antibody in Jurkat (green) compare to negative control of rabbit IgG (blue)

Western Blot Analysis

Western Blot analysis of Jurkat cell lysate with CD3 antibody

Product Details:

Technology	Rabbit Monoclonal
Clone Name	SP162
Application	IHC-P, WB, FACS
Species Reactivity
Tested	Hu
Predicted by homology	Bv, Ck, Dg, Ms, Pg, Rb, Rt
Isotype	Rabbit IgG
Immunogen	Synthetic peptide corresponding to internal region of the epsilon chain of human CD3 protein.
Cellular Localization	Membrane
Control Tissue	Tonsil
Regulatory Rating	Research Use Only (RUO)
Entrez	916
Storage Requirement	2-8°C
Target Molecular Weight	20 kDa
Formulation	Refer to Data Sheet

Associated Products

Rabbit Anti-Human CD3 Monoclonal Antibody (Clone SP7) Read more

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How to retrieve a certificate of analysis

Rabbit Anti-Human CD3 Monoclonal Antibody (Clone SP162)

Associated Products