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Rabbit Anti-Human CD3 Monoclonal Antibody (Clone SP162)

For research use only. Not for use in diagnostics.

Product No. Pack Size
06975020001 0.1 mL
Product No. Pack Size
06975046001 1.0 mL

To order these products, please contact your Roche order management team.

This antibody reacts with the intracytoplasmic portion of the CD3 antigen expressed by T cells. It stains human T cells in both the cortex and medulla of the thymus and in peripheral lymphoid tissues. This antibody is suitable for staining normal and neoplastic T cells in formalin-fixed, paraffin-embedded tissues.

Figure above:
Human Tonsil stained with anti-CD3 antibody

Contents

Rabbit monoclonal antibody purified by protein A/G in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.

IHC Performance

 

Human B-cell lymphoma stained with anti-CD3 antibody

Human Bladder stained with anti-CD3 antibody

Human Cervix stained with anti-CD3 antibody

Human Colon stained with anti-CD3 antibody

Human B-cell lymphoma stained with anti-CD3 antibody

Human Bladder stained with anti-CD3 antibody

Human Cervix stained with anti-CD3 antibody

Human Colon stained with anti-CD3 antibody

Human Esophagus stained with anti-CD3 antibody

Human Fallopian Tube stained with anti-CD3 antibody

Human HK lymphoma stained with anti-CD3 antibody

Human Liver stained with anti-CD3 antibody

Human Esophagus stained with anti-CD3 antibody

Human Fallopian Tube stained with anti-CD3 antibody

Human HK lymphoma stained with anti-CD3 antibody

Human Liver stained with anti-CD3 antibody

Human Spleen stained with anti-CD3 antibody

Human Thymus stained with anti-CD3 antibody

Human Tonsil stained with anti-CD3 antibody

Human Spleen stained with anti-CD3 antibody

Human Thymus stained with anti-CD3 antibody

Human Tonsil stained with anti-CD3 antibody



Flow Cytometric Analysis

 

Flow cytometric analysis of rabbit anti-CD3 (SP162) antibody in Jurkat (green) compare to negative control of rabbit IgG (blue)

Flow cytometric analysis of rabbit anti-CD3 (SP162) antibody in Jurkat (green) compare to negative control of rabbit IgG (blue)



Western Blot Analysis

 

Western Blot analysis of Jurkat cell lysate with CD3 antibody

Western Blot analysis of Jurkat cell lysate with CD3 antibody

Product Details:

 

Technology

Rabbit Monoclonal

Clone Name

SP162

Application

IHC-P, WB, FACS

Species Reactivity

 

   Tested

Hu

   Predicted by homology

Bv, Ck, Dg, Ms, Pg, Rb, Rt

Isotype

Rabbit IgG

Immunogen

Synthetic peptide corresponding to internal region of the epsilon chain of human CD3 protein.

Cellular Localization

Membrane

Control Tissue

Tonsil

Regulatory Rating

Research Use Only (RUO)

Entrez

916

Storage Requirement

2-8°C

Target Molecular Weight

20 kDa

Formulation

Refer to Data Sheet