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Rabbit Anti-Human CD43 Monoclonal Antibody (Clone SP55)

For research use only. Not for use in diagnostics.

Product No. Pack Size
06970982001 0.1 mL
Product No. Pack Size
06971024001 1.0 mL

To order these products, please contact your Roche order management team.

CD43 is one of the major glycoproteins of thymocytes and T lymphocytes. It plays a role in the physicochemical properties of the T cell surface and in lectin binding. CD43 presents carbohydrate ligands to selectins. It has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. The antigen is a counter receptor for SN/Siglec1. During T cell activation CD43 is actively removed from the T cell antigen presenting cell contact site suggesting a negative regulatory role in adaptive immune response. **Please contact us for availability**

Figure above:
Human Tonsil stained with anti-CD43 antibody

Contents

Rabbit monoclonal antibody purified by protein A/G in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.

IHC Performance

 

Human Tonsil stained with anti-CD43 antibody

Human Bladder Transitional Cell Carcinoma stained with anti-CD43 antibody

Human Thymus stained with anti-CD43 antibody

Human Spleen stained with anti-CD43 antibody

Human Tonsil stained with anti-CD43 antibody

Human Bladder Transitional Cell Carcinoma stained with anti-CD43 antibody

Human Thymus stained with anti-CD43 antibody

Human Spleen stained with anti-CD43 antibody.

Human Prostate stained with anti-CD43 antibody

Human Esophagus stained with anti-CD43 antibody

Human Endometrium stained with anti-CD43 antibody

Human Colon stained with anti-CD43 antibody

Human Prostate stained with anti-CD43 antibody

Human Esophagus stained with anti-CD43 antibody

Human Endometrium stained with anti-CD43 antibody

Human Colon stained with anti-CD43 antibody

Human Colon Adenocarcinoma stained with anti-CD43 antibody

Human Lung stained with anti-CD43 antibody

Human Colon Adenocarcinoma stained with anti-CD43 antibody

Human Lung stained with anti-CD43 antibody

 

Flow cytometric analysis

 

Flow cytometric analysis of rabbit anti-CD43 (SP55) antibody in Jurkat (green) compare to negative control of rabbit IgG (blue)
Flow cytometric analysis of rabbit anti-CD43 (SP55) antibody in Jurkat (green) compare to negative control of rabbit IgG (blue)

 

Western Blot analysis

 

Western Blot of of rabbit anti-CD43 (SP55) antibody
Western Blot of of rabbit anti-CD43 (SP55) antibody

Product Details:

 

Technology

Rabbit Monoclonal

Clone Name

SP55

Application

IHC-P, WB, FACS

Species Reactivity

 

   Tested

Hu

   Predicted by homology

N/A

Isotype

Rabbit IgG

Immunogen

Synthetic peptide derived from the human CD43.

Cellular Localization

Membrane

Control Tissue

Tonsil

Regulatory Rating

Research Use Only (RUO)

Entrez

6693

Storage Requirement

2-8°C

Target Molecular Weight

95/115/135 kDa

Formulation

Refer to Data Sheet