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RealTime ready Pre-Amp Primer Pool

For life science research only. Not for use in diagnostic procedures.

Product No. Pack Size
06725309001 40 reactions

To order these products, please contact your Roche order management team.

  • Select and order a custom primer pool for your RealTime ready Custom Panel.
  • High quality primer pairs, ensure a virtually bias-free target specific pre-amplification.
  • Ideally suited for pre-amplification of cDNA derived from RNA isolated from formalin-fixed, paraffin-embedded tissue samples. 

Contents

RealTime ready cDNA Pre-Amp Primer Pool, 4× concentrated

The RealTime ready Pre-Amp Primer Pool contains a mixture of primers from custom selected RealTime ready Assays in a ready-to-use concentration for cDNA multiplex cDNA pre-amplification PCR. It is used with the RealTime ready cDNA Pre-Amp Master. Pre-amplified cDNA is then applied to the corresponding RealTime ready Custom Panel. The primer pool is customized by configuring a RealTime ready Panel on the online portal of the RealTime ready Configurator.
Note: RealTime ready Pre-Amp Primer Pool containing Designer Assays must be ordered along with the corresponding RealTime ready Custom Panel.

Principle

cDNA is pre-amplified in a multiplex PCR using custom RealTime ready Pre-Amp Primer Pools containing 6 to 96 specific primer pairs corresponding to the primers that you require for RealTime ready Assays you have selected.
The resulting product is a uniform doublestranded pre-amplification of your selected targets, without the bias that can be produced using whole transcriptome amplification technologies. This pre-amplified cDNA is ideal for use with pre-plated, function tested RealTime ready Assays.