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Detection of Classical Swine Fever with the LightCycler Instrument
Easy and Reliable qRT-PCR Analysis of Total RNA Isolated from Fresh Frozen and FFPE Tissue Samples
Here, we present data for two independent workflows in gene expression analysis using Roche’s LightCycler® 480 Instrument.
Comparison of Automated MagNA Pure 96 and Manual PAXgene RNA Isolation from Stabilized Peripheral Blood Samples
Experiments in this paper investigate the quality and capacity of RNA isolation from PAXgene tubes on the MagNA Pure 96 System compared to conventional manual extraction methods. Researchers used a modified RNA isolation protocol (described in the MagNA Pure 96 Cellular RNA Large Volume Kit product insert) that extracts up to 48 stabilized blood samples (or 96 aliquots) simultaneously. Attributes such as purity, recovery, RNA integrity (RIN) and sensitivity in downstream RT-PCR applications were compared and analyzed. The goal for these studies is to develop an optimized and more efficient workflow for the detection of CRC biomarkers in peripheral blood samples.