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Conforms to the European directive for in vitro diagnostic medical devices 98/79/EC. For USA: For general laboratory use.
High-throughput robotic workstation for fully automated purification of nucleic acids from up to 96 samples.
For in vitro diagnostic use.
Comparison of Automated MagNA Pure 96 and Manual PAXgene RNA Isolation from Stabilized Peripheral Blood Samples
Experiments in this paper investigate the quality and capacity of RNA isolation from PAXgene tubes on the MagNA Pure 96 System compared to conventional manual extraction methods. Researchers used a modified RNA isolation protocol (described in the MagNA Pure 96 Cellular RNA Large Volume Kit product insert) that extracts up to 48 stabilized blood samples (or 96 aliquots) simultaneously. Attributes such as purity, recovery, RNA integrity (RIN) and sensitivity in downstream RT-PCR applications were compared and analyzed. The goal for these studies is to develop an optimized and more efficient workflow for the detection of CRC biomarkers in peripheral blood samples.
Detection of Classical Swine Fever with the LightCycler Instrument
Easy and Reliable qRT-PCR Analysis of Total RNA Isolated from Fresh Frozen and FFPE Tissue Samples
Here, we present data for two independent workflows in gene expression analysis using Roche’s LightCycler® 480 Instrument.
MagNA Pure 96 System
Detection of bacterial, fungal and viral NA in routine microbiological sample types on the MagNA Pure 96 System
The objective of this study was to compare the MagNA Pure 96 Pathogen Universal protocol to the MagNA Pure LC DNA Isolation Kit III that is well established in our microbiological laboratory. For this we isolated nucleic acids from ten different sample materials: body fluids (CSF, urine, sputum), feces, swabs, as well as whole blood, EDTA-, Citrate-plasma and serum. This spectrum is typical for a microbiology laboratory and includes easy and difficult-to-process samples, as sputum and stool are. Into these sample types we spiked classifi ed pathogen stocks from in-house cultures. Subsequently real-time PCR analysis of fungal, bacterial and viral targets was done using the LightCycler® 480 Instrument.