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Total Search results for "High Resolution Melting" ()

To order these products, please contact your Roche order management team.

Products (2)

LightCycler® 480 High Resolution Melting Dye

Fluorescent dye for detection of double-stranded DNA in real-time PCR and high resolution melting analysis.

For life science research only. Not for use in diagnostic procedures.

LightCycler® 480 High Resolution Melting Master

Ready-to-use hot start reaction mix for real-time PCR and high resolution melting curve analysis using the LightCycler® 480, LightCycler® 96 and LightCycler® Nano Instrument.

For life science research only. Not for use in diagnostic procedures.

Publications (8)

Methylation-sensitive high-resolution melting 

Keywords: Lightcycler 480 ,Lightcycler 480 ResoLight Dye ,Taq polymerase ,Light Cycler 480 HRM Master ,Resolight Dye

T. Wojdacz , A. Dobrovic and L. Hansen

Nature Protocols, 3, 1903 - 1908

A rapid high-throughput method for the detection and quantification of RNA editing based on high-resolution melting of amplicons 

Keywords: Lightcycler 480 ,Lightcycler 480 Probes Master Mix

A. Chateigner-Boutin and I. Small

Nucleic Acids Res, 35, 17, e114 -

Microsatellite Analysis of Grapevine Varieties by High-Resolution Amplicon Melting Analysis

 

Keywords: LightCycler 480 Gene Scanning Software ,LightCycler 480 High Resolution Melting Master

J. Mackay , C. Wright and R. Bonfiglioli

Biochemica, 2, 19-20

Identification of ryanodine receptor 1 single-nucleotide polymorphisms by high-resolution melting using the LightCycler 480 System 

Keywords: Lightcycler 480 ,LightCycler probe design software

Hilbert G. and Kathryn S.

Anal Biochem, 374, 2, 396 - 404

Detection of High-Frequency and Novel DNMT3A Mutations in Acute Myeloid Leukemia by High-Resolution Melting Curve Analysis 

Keywords: LightCycler480 ,Genotyping ,HRM ,Cancer Research

R. R. Singh , A. Bains , K. P. Patel , H. Rahimi , B. A. Barkoh , A. Paladugu , T. Bisrat , F. Ravandi-Kashan , J. E. Cortes , H. M. Kantarjian , L. .J.Medeiross , R. Luthra

The Journal of Molecular Diagnostics, 14, 4, Pages 336-345

Documents (15)

Rapid High-Throughput Methylation Analysis Using the LightCycler® 480 System

We assessed the performance of the instrument combined with the LightCycler® 480 High Resolution Melting Master mix for DNA methylation analysis using the methylation-sensitive high resolution melting (MS-HRM) methodology.

(["Article"], PDF, 177 KB) Download

LightCycler® 480 Real-Time PCR System - High Resolution Melting: Optimization Strategies

High resolution melting (HRM) is a novel, closed-tube, post-PCR technique allowing genomic researchers to easily analyze genetic variations in PCR amplicons.

(["Article"], PDF, 3386 KB) Download

Rapid and Reliable Methylation Detection in Archival Tissue Samples using High-Resolution Melting Analysis

The aim of the present study was to establish and evaluate HRM assays for detection of promoter methylation on archival FFPE tissues from individuals with colorectal cancer.

(["Article"], PDF, 986 KB) Download

Mutation Scanning of the Cytidine Deaminase Gene by High-Resolution Melting Curve Analysis Using the LightCycler® 480 System

Our results show that the LightCycler® 480 System provides a novel, integrated solution to scanning gene fragments for sequence variations based on post-PCR melting curve analysis at high resolutions.

(["Article"], PDF, 194 KB) Download

Mutation Scanning Using High Resolution Melting or dHPLC: a Performance Comparison Study

Very little effort is required to replace dHPLC Systems with the LightCycler® 480 System for mutation scanning and detection in biomedical research and testing.

(["Article"], PDF, 207 KB) Download
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