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LightCycler® RNA Amplification Kit HybProbe
Kit for one-step RT-PCR using the LightCycler® Carousel-Based System.
For general laboratory use.
LightCycler® RNA Master HybProbe
Easy-to-use reaction mix for one-step RT-PCR using the LightCycler® Carousel-Based System.
For general laboratory use.
LightCycler® FastStart DNA MasterPLUS HybProbe
Easy-to-use hot start reaction mix for PCR using the LightCycler® Carousel-Based System.
For general laboratory use.
LightCycler® DNA Master HybProbe
Easy-to-use fast high-performance qPCR kit for HybProbe signal transmission with all LightCycler® Carousel-Based Instruments.
For general laboratory use.
LightCycler® FastStart DNA Master HybProbe
Easy-to-use hot start reaction mix for PCR using the LightCycler® Carousel-Based System.
For general laboratory use.
LightCycler® Hybridization Probes
The most direct way to monitor PCR amplification for quantification and mutation detection.
PNA Clamp Technique for Detecting a Ki-ras2 Mutation Using the LightCycler Instrument
Literature indicates that point mutations in codon 12 of the Ki-ras2 gene are associated with colon cancer. The detection of a point mutation in the high background of wild-type cells is very difficult, which represents a problem for many research projects focused on processes that take place during cancerogenesis. Therefore, a quick and easy, yet reliable method of detecting single point mutations is preferred. This article shows an application example for Peptide Nucleic Acid (PNA) oligomers in combination with Hybridization Probes on the LightCycler Instrument. Here, PNAs suppress the codon 12 wild-type PCR product, as they bind to nucleic acids with higher stringency, compared to deoxyribonucleotides, and do not serve as primers for Taq polymerase. Due to these properties, PNAs can be used to detect single point mutations in research samples in a high background of wild-type sequences.
Rapid Genotyping and Quantification on the LightCyclerTM with Hybridization Probes
Simultaneous Detection of SNPs in Four Porcine Genes Using Hybridization Probes and the LightCycler<® 2.0 Instrument
Identification of Cytokine SNPs Using LightCycler Hybridization Probes and Melting Curve Analysis
The analysis of single nucleotide polymorphisms (SNPs) in cytokine genes has begun to shed some light on the roles of cytokines in the pathophysiology of various diseases. Allelic SNPs in the promoter region of interleukin 10 (IL-10) and tumor necrosis factor alpha (TNF-?) genes can affect the respective levels of cytokine produced.
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