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Total Search results for "Hybridization Probes" ()

To order these products, please contact your Roche order management team.

Products (7)

LightCycler® RNA Amplification Kit HybProbe

Kit for one-step RT-PCR using the LightCycler® Carousel-Based System. 

For general laboratory use.

LightCycler® RNA Master HybProbe

Easy-to-use reaction mix for one-step RT-PCR using the LightCycler® Carousel-Based System. 

For general laboratory use.

LightCycler® FastStart DNA MasterPLUS HybProbe

Easy-to-use hot start reaction mix for PCR using the LightCycler® Carousel-Based System.

For general laboratory use.

LightCycler® FastStart DNA Master HybProbe

Easy-to-use hot start reaction mix for PCR using the LightCycler® Carousel-Based System.

For general laboratory use.

LightCycler® DNA Master HybProbe

Easy-to-use fast high-performance qPCR kit for HybProbe signal transmission with all LightCycler® Carousel-Based Instruments.

For general laboratory use.

Publications (5)

Automated RNA Extraction by MagNA Pure Followed by Rapid Quantification of Cytokine and Chemokine Gene Expression with Use of Fluorescence Resonance Energy Transfer 

Keywords: MagNA Pure LC ,LightCycler system ,MagNA Pure LC RNA Isolation Kit I ,Transcriptor First Strand cDNA Synthesis Kit ,LightCycler FastStart DNA Master Hybridization Probes

J. Loeffler , P. Swatoch , D. Akhawi-Araghi , H. Hebart and H. Einsele

Clin Chem , 49, 6, 955-958

Rapid quantitative detection of TE-AML1 fusion transcripts in pediatric acute lymphoblastic leukemia by real-time reverse transcription polymerase chain reaction using fluorescently labeled probes 

Keywords: LightCycler DNA Master Hybridization Probes ,LightCycler FastStart DNA Master Hybridization Probes

P. Bolufer , E. Barragan , A. Verdeguer , J. Cervera , J. M. Fernandez , I. Moreno , E. Lerma , C. Esquembre , M. Tasso , V. Fuster , M. Bermudez and M. Sanz

Haematologica, 87, 1, 23 - 32

High-throughput detection of bacterial, fungal and viral nucleic acids in routine microbiology sample types using one generic Pathogen Universal Protocol on the MagNA Pure 96 System

 

Keywords: MagNA Pure 96 ,LightCycler 480 ,LightCycler FastStart DNA Master Hybridization Probes

M. Kirchgesser , A. Aschenbrenner , M. Bollwein , U. Reischl

Application Note 5

Multiplex LightCycler PCR Assay for Detection and Differentiation of Bordetella pertussis and Bordetella parapertussis in Nasopharyngeal Specimens 

Keywords: LightCycler Carousel ,PCR Nucleotide Mix PLUS ,Hybridization Probes

L. Sloan , M. Hopkins , P. S. Mitchell , E. Vetter , J. Rosenblatt , W. S. Harmsen , F. Cockerill and R. Patel

J Clin Microbiol, 40, 1, 96 - 100

Documents (11)

LightCycler® Hybridization Probes

The most direct way to monitor PCR amplification for quantification and mutation detection.

(["Article"], PDF, 2373 KB) Download

PNA Clamp Technique for Detecting a Ki-ras2 Mutation Using the LightCycler Instrument

Literature indicates that point mutations in codon 12 of the Ki-ras2 gene are associated with colon cancer. The detection of a point mutation in the high background of wild-type cells is very difficult, which represents a problem for many research projects focused on processes that take place during cancerogenesis. Therefore, a quick and easy, yet reliable method of detecting single point mutations is preferred. This article shows an application example for Peptide Nucleic Acid (PNA) oligomers in combination with Hybridization Probes on the LightCycler Instrument. Here, PNAs suppress the codon 12 wild-type PCR product, as they bind to nucleic acids with higher stringency, compared to deoxyribonucleotides, and do not serve as primers for Taq polymerase. Due to these properties, PNAs can be used to detect single point mutations in research samples in a high background of wild-type sequences.

(["Article"], PDF, 282 KB) Download

Identification of Cytokine SNPs Using LightCycler Hybridization Probes and Melting Curve Analysis

The analysis of single nucleotide polymorphisms (SNPs) in cytokine genes has begun to shed some light on the roles of cytokines in the pathophysiology of various diseases. Allelic SNPs in the promoter region of interleukin 10 (IL-10) and tumor necrosis factor alpha (TNF-?) genes can affect the respective levels of cytokine produced.

(["Article"], PDF, 319 KB) Download

Simultaneous Detection of SNPs in Four Porcine Genes Using Hybridization Probes and the LightCycler 2.0 Instrument

(["Article"], PDF, 262 KB) Download

Rapid Genotyping and Quantification on the LightCyclerTM with Hybridization Probes

(["Article"], PDF, 404 KB) Download
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