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Products (100)

LightCycler® 480 Block Kit 96 Silver

Easily interchangeable 96-well thermal block cycler unit for the LightCycler® 480 Instrument.

For life science research only. Not for use in diagnostic procedures.

LightCycler® 480 Block Kit 384 Silver

Easily interchangeable 384-well thermal block cycler unit for the LightCycler® 480 Instrument.

For life science research only. Not for use in diagnostic procedures.

LightCycler® 480 Instrument II

Rapid high-throughput, plate-based real-time PCR amplification and detection instrument.

For life science research only. Not for use in diagnostic procedures.

LightCycler® 8-Tube Strips (white)

Disposable  8-Tube Strips for the LightCycler® 96 Instrument and LightCycler® 480 Instrument.

For life science research only. Not for use in diagnostic procedures.

RealTime ready Custom Panel 384 - 96

Function tested RT-qPCR assays for human, mouse, or rat genes of your choice, preplated on LightCycler® 480 Multiwell Plates, 384.

For life science research only. Not for use in diagnostic procedures.

Documents (47)

01_01_44444_bc_2_06_title.pdf

In this study, the LightCycler® 480 Instrument was evaluated for genotyping at high throughputs, based on melting temperature differences observed when different alleles are recognized with HybProbe probes.

(["Article"], PDF, 216 KB) Download

Utility of the MagNA Pure 96 and LightCycler® 480 System for the Detection of Respiratory Pathogens

Acute respiratory disease (ARD) is a major cause of morbidity in developed countries and accounts for a large number of hospitalizations of especially young children. In addition, in developing countries, it constitutes an important cause of death. Viruses were shown to be a major cause of ARD. The last decade has seen the growing importance of molecular techniques in not only the discovery of novel viral respiratory pathogens, but also in routine testing of viral respiratory disease, replacing traditional virus detection methods in many laboratories. Also in our laboratory, a multiplex real-time PCR respiratory panel was introduced a number of years ago, leading to faster analysis and detection. In 2010, the MagNA Pure 96 System was introduced into the laboratory, where it is combined with the LightCycler® 480 Instrument to yield an easy-touse, efficient set-up for molecular research. This paper describes our results obtained with this setup in analysis of viral respiratory samples in 2010 and 2011.

(["Article"], PDF, 650 KB) Download

Utility of the MagNA Pure 96 and LightCycler® 480 Systems for Testing of Stool Samples for Toxin-producing C. Difficile

Clostridium difficile infection (CDI) is an important cause of hospital diarrhea. Conventional CDI testing by enzyme immunoassays is fast yet shows poor sensitivity and specificity, whereas the "gold standard" toxigenic culture is laborious and time consuming. Molecular tests for CDI are fast and highly sensitive and specific. Our laboratory has developed a molecular procedure that allows for rapid detection of CDI directly in stool samples. It is based on the isolation of highly pure DNA extracts from feces using the MagNA Pure 96 System, followed by detection of the C. difficile toxins tcdA and tcdB using real-time PCR on the LightCycler®480 Instrument.

(["Article"], PDF, 935 KB) Download

Easy and Reliable qRT-PCR Analysis of Total RNA Isolated from Fresh Frozen and FFPE Tissue Samples

Here, we present data for two independent workflows in gene expression analysis using Roche’s LightCycler® 480 Instrument.

(["Article"], PDF, 848 KB) Download

Fast qRT-PCR with LightCycler® 480 RNA Master Hydrolysis Probes

This kit is an easy-to-use hot-start reaction mix, specifically adapted for one-step RT-PCR under the rapid and accurate cycling conditions of the LightCycler® 480 Instrument using hydrolysis probes as detection format.

(["Article"], PDF, 138 KB) Download
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