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Total Search results for "LightCycler 480 Instrument" ()

To order these products, please contact your Roche order management team.

Products (100)

LightCycler® 480 Block Kit 96 Silver

Easily interchangeable 96-well thermal block cycler unit for the LightCycler® 480 Instrument.

For life science research only. Not for use in diagnostic procedures.

LightCycler® 480 Block Kit 384 Silver

Easily interchangeable 384-well thermal block cycler unit for the LightCycler® 480 Instrument.

For life science research only. Not for use in diagnostic procedures.

LightCycler® 480 Instrument II

Rapid high-throughput, plate-based real-time PCR amplification and detection instrument.

For life science research only. Not for use in diagnostic procedures.

LightCycler® 8-Tube Strips (white)

Disposable  8-Tube Strips for the LightCycler® 96 Instrument and LightCycler® 480 Instrument.

For life science research only. Not for use in diagnostic procedures.

RealTime ready Custom Panel 384 - 96

Function tested RT-qPCR assays for human, mouse, or rat genes of your choice, preplated on LightCycler® 480 Multiwell Plates, 384.

For life science research only. Not for use in diagnostic procedures.

Documents (47)

01_01_44444_bc_2_06_title.pdf

In this study, the LightCycler® 480 Instrument was evaluated for genotyping at high throughputs, based on melting temperature differences observed when different alleles are recognized with HybProbe probes.

(["Article"], PDF, 216 KB) Download

Utility of the MagNA Pure 96 and LightCycler® 480 System for the Detection of Respiratory Pathogens

Acute respiratory disease (ARD) is a major cause of morbidity in developed countries and accounts for a large number of hospitalizations of especially young children. In addition, in developing countries, it constitutes an important cause of death. Viruses were shown to be a major cause of ARD. The last decade has seen the growing importance of molecular techniques in not only the discovery of novel viral respiratory pathogens, but also in routine testing of viral respiratory disease, replacing traditional virus detection methods in many laboratories. Also in our laboratory, a multiplex real-time PCR respiratory panel was introduced a number of years ago, leading to faster analysis and detection. In 2010, the MagNA Pure 96 System was introduced into the laboratory, where it is combined with the LightCycler® 480 Instrument to yield an easy-touse, efficient set-up for molecular research. This paper describes our results obtained with this setup in analysis of viral respiratory samples in 2010 and 2011.

(["Article"], PDF, 650 KB) Download

Utility of the MagNA Pure 96 and LightCycler® 480 Systems for Testing of Stool Samples for Toxin-producing C. Difficile

Clostridium difficile infection (CDI) is an important cause of hospital diarrhea. Conventional CDI testing by enzyme immunoassays is fast yet shows poor sensitivity and specificity, whereas the "gold standard" toxigenic culture is laborious and time consuming. Molecular tests for CDI are fast and highly sensitive and specific. Our laboratory has developed a molecular procedure that allows for rapid detection of CDI directly in stool samples. It is based on the isolation of highly pure DNA extracts from feces using the MagNA Pure 96 System, followed by detection of the C. difficile toxins tcdA and tcdB using real-time PCR on the LightCycler® 480 Instrument.

(["Article"], PDF, 935 KB) Download

Rapid High-Throughput Methylation Analysis Using the LightCycler® 480 System

We assessed the performance of the instrument combined with the LightCycler® 480 High Resolution Melting Master mix for DNA methylation analysis using the methylation-sensitive high resolution melting (MS-HRM) methodology.

(["Article"], PDF, 177 KB) Download

Transfering PCRs to HRM-assays on the LightCycler® 480 System - Examples for BRCA1

To evaluate how easy it is to design hrMCA assays using the LightCycler® 480 Instrument, we selected 3 different gene fragments, designed an MCA assay, and tested its sensitivity to detect known variants.

(["Article"], PDF, 248 KB) Download
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