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High-throughput robotic workstation for fully automated purification of nucleic acids from up to 96 samples.
For in vitro diagnostic use.
A fully-automated clinical nucleic acid extraction system that brings you walkaway automation for up to 24 samples.
For in vitro diagnostic use.
Use of the Roche LightCycler Strep B Assay for Detection of Group B Streptococcus from Vaginal and Rectal Swabs
J Clin Microbiol, 43, 8, 4046 - 4051
Background: Peripheral blood based molecular diagnostics requires automated sample processing and evaluation of RNA for RT-PCR analysis. Our aims were to compare the automated and manual RNA isolation and to determine the purity, recovery, RNA integrity (RIN) and sensitivity of downstream RT-PCR applications.
Methods: Total RNA was isolated from blood samples of 25 individuals with colorectal cancer (CRC) and 19 healthy individuals, either manually using PAXgene Blood RNA Kit and in parallel, using the MagNA Pure 96 Instrument. The expression of seven CRC and one adenoma markers was analyzed using real-time RT-PCR.
Results: OD260/280 of manually isolated samples (2.09) was equal to that of MagNA Pure 96 Instrument isolated samples (2.01), while OD230/260 of MagNA Pure 96 Instrument samples (1.85) was signifi cantly better than that of manually isolated samples (1.52). The RIN is acceptable in case of both isolation methods. The manual protocol produced slightly more intact RNA (RIN-manual: 9.19 +/- 0.26; RIN-MagNA Pure 96 Instrument: 8.25). The MagNA Pure 96 System resulted in slightly higher yields of isolated RNA (manual: 5.65 μg/tube; MagNA Pure 96 System: 5.99 μg/tube). Marker correlation between the two batches isolated using the MagNA Pure 96 Instrument and between two PAXgene batches was high. In overall qPCR reproducibility and mRNA yield/ratio were higher with the MagNA Pure 96 System. The automated method showed less standard deviation. Genomic DNA contamination could not be detected in any of the MagNA Pure 96 System isolated RNA samples.
Conclusion: Automated RNA isolation from peripheral blood samples using the MagNA Pure 96 Instrument produces high quality, high purity and high yield total RNA. MagNA Pure 96 Instrument extraction is a fully automated, fast and reliable method with 96 extractions in 85 min, compared to the equally precise, but time consuming manual extraction.
Automated Extraction of Genomic DNA from Medically Important Yeast Species and Filamentous Fungi by Using the MagNA Pure LC System
J Clin Microbiol, 40, 6, 2240 - 2243
Comparison of Automated MagNA Pure 96 and Manual PAXgene RNA Isolation from Stabilized Peripheral Blood Samples
Experiments in this paper investigate the quality and capacity of RNA isolation from PAXgene tubes on the MagNA Pure 96 System compared to conventional manual extraction methods. Researchers used a modified RNA isolation protocol (described in the MagNA Pure 96 Cellular RNA Large Volume Kit product insert) that extracts up to 48 stabilized blood samples (or 96 aliquots) simultaneously. Attributes such as purity, recovery, RNA integrity (RIN) and sensitivity in downstream RT-PCR applications were compared and analyzed. The goal for these studies is to develop an optimized and more efficient workflow for the detection of CRC biomarkers in peripheral blood samples.
Two New Protocols for the MagNA Pure Compact System: DNA_Blood_external_lysis and Total_NA_external_lysis
In this Application we compare nucleic acids prepared with two new isolation protocols for the MagNA Pure Compact Instrument to nucleic acids prepared with two existing MagNA Pure Compact Protocols.
Utility of the MagNA Pure 96 and LightCycler® 480 Systems for Testing of Stool Samples for Toxin-producing C. Difficile
Clostridium difficile infection (CDI) is an important cause of hospital diarrhea. Conventional CDI testing by enzyme immunoassays is fast yet shows poor sensitivity and specificity, whereas the "gold standard" toxigenic culture is laborious and time consuming. Molecular tests for CDI are fast and highly sensitive and specific. Our laboratory has developed a molecular procedure that allows for rapid detection of CDI directly in stool samples. It is based on the isolation of highly pure DNA extracts from feces using the MagNA Pure 96 System, followed by detection of the C. difficile toxins tcdA and tcdB using real-time PCR on the LightCycler® 480 Instrument.
Detection of bacterial, fungal and viral NA in routine microbiological sample types on the MagNA Pure 96 System
The objective of this study was to compare the MagNA Pure 96 Pathogen Universal protocol to the MagNA Pure LC DNA Isolation Kit III that is well established in our microbiological laboratory. For this we isolated nucleic acids from ten different sample materials: body fluids (CSF, urine, sputum), feces, swabs, as well as whole blood, EDTA-, Citrate-plasma and serum. This spectrum is typical for a microbiology laboratory and includes easy and difficult-to-process samples, as sputum and stool are. Into these sample types we spiked classifi ed pathogen stocks from in-house cultures. Subsequently real-time PCR analysis of fungal, bacterial and viral targets was done using the LightCycler® 480 Instrument.
Utility of the MagNA Pure 96 and LightCycler® 480 System for the Detection of Respiratory Pathogens
Acute respiratory disease (ARD) is a major cause of morbidity in developed countries and accounts for a large number of hospitalizations of especially young children. In addition, in developing countries, it constitutes an important cause of death. Viruses were shown to be a major cause of ARD. The last decade has seen the growing importance of molecular techniques in not only the discovery of novel viral respiratory pathogens, but also in routine testing of viral respiratory disease, replacing traditional virus detection methods in many laboratories. Also in our laboratory, a multiplex real-time PCR respiratory panel was introduced a number of years ago, leading to faster analysis and detection. In 2010, the MagNA Pure 96 System was introduced into the laboratory, where it is combined with the LightCycler® 480 Instrument to yield an easy-touse, efficient set-up for molecular research. This paper describes our results obtained with this setup in analysis of viral respiratory samples in 2010 and 2011.
Roche Life Science | MagNA Pure 24 System
The new MagNA Pure 24 System offers automated extraction of 1 to 24 samples with primary sample handling in just over an hour, using a single universal reagent kit covering 10 human samples types.