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Total Search results for "MagNA Pure 96 Instrument" ()

To order these products, please contact your Roche order management team.

Products (33)

MagNA Pure 96 Instrument

High-throughput robotic workstation for fully automated purification of nucleic acids from up to 96 samples.

For in vitro diagnostic use.

MagNA Pure 96 DNA and Viral NA Large Volume Kit

Ready-to-use reagents in two prefilled sealed trays for purifying nucleic acids from up to 1,000 μL sample volume using the MagNA Pure 96 Instrument.
For in vitro diagnostic use.

MagNA Pure 96 DNA and Viral NA Small Volume Kit

Ready-to-use reagents in two prefilled sealed trays for purifying nucleic acids from up to 200 μL sample volumes using the MagNA Pure 96 Instrument.

For in vitro diagnostic use.

 

MagNA Pure 96 System Fluid (Internal)

For in vitro diagnostic use.

MagNA Pure 96 System Fluid (External)

For in vitro diagnostic use.

Publications (3)

Comparison of Automated MagNA Pure 96 and Manual PAXgene RNA Isolation from Stabilized Peripheral Blood Samples

 

Keywords: MagNA Pure 96

O. Galamb , K. Leiszter , B. Wichmann , S. Spisak , A. Kalmar , K. Toth , B. Molnar

Application Note 2

High-throughput detection of bacterial, fungal and viral nucleic acids in routine microbiology sample types using one generic Pathogen Universal Protocol on the MagNA Pure 96 System

 

Keywords: MagNA Pure 96 ,LightCycler 480 ,LightCycler FastStart DNA Master Hybridization Probes

M. Kirchgesser , A. Aschenbrenner , M. Bollwein , U. Reischl

Application Note 5

Use of MagNA Pure 96 and LightCycler® Systems for High Throughput Testing of Stool Samples for enterohemorrhagic E. coli (EHEC) in an Outbreak

 

Keywords: MagNA Pure 96 ,LightCycler 480 ,Hybridization Probe

R. Bialek , O. Landt , M. Bollwein , U. Reischl

Application Note 3

Documents (17)

E. coli (EHEC) Extraction and Detection in Stool Samples through Use of MagNA Pure 96 System and LightCycler® Instrument

To detect the EHEC bacterium, the automated MagNA Pure 96 System for nucleic acid isolation and LightCycler® * Real-Time PCR Instrument (Roche Diagnostics) were used for high throughput examination of EHEC directly in stool samples.

(["Article"], PDF, 1246 KB) Download

Utility of the MagNA Pure 96 and LightCycler® 480 Systems for Testing of Stool Samples for Toxin-producing C. Difficile

Clostridium difficile infection (CDI) is an important cause of hospital diarrhea. Conventional CDI testing by enzyme immunoassays is fast yet shows poor sensitivity and specificity, whereas the "gold standard" toxigenic culture is laborious and time consuming. Molecular tests for CDI are fast and highly sensitive and specific. Our laboratory has developed a molecular procedure that allows for rapid detection of CDI directly in stool samples. It is based on the isolation of highly pure DNA extracts from feces using the MagNA Pure 96 System, followed by detection of the C. difficile toxins tcdA and tcdB using real-time PCR on the LightCycler® 480 Instrument.

(["Article"], PDF, 935 KB) Download

Utility of the MagNA Pure 96 and LightCycler® 480 System for the Detection of Respiratory Pathogens

Acute respiratory disease (ARD) is a major cause of morbidity in developed countries and accounts for a large number of hospitalizations of especially young children. In addition, in developing countries, it constitutes an important cause of death. Viruses were shown to be a major cause of ARD. The last decade has seen the growing importance of molecular techniques in not only the discovery of novel viral respiratory pathogens, but also in routine testing of viral respiratory disease, replacing traditional virus detection methods in many laboratories. Also in our laboratory, a multiplex real-time PCR respiratory panel was introduced a number of years ago, leading to faster analysis and detection. In 2010, the MagNA Pure 96 System was introduced into the laboratory, where it is combined with the LightCycler® 480 Instrument to yield an easy-touse, efficient set-up for molecular research. This paper describes our results obtained with this setup in analysis of viral respiratory samples in 2010 and 2011.

(["Article"], PDF, 650 KB) Download

Detection of bacterial, fungal and viral NA in routine microbiological sample types on the MagNA Pure 96 System

The objective of this study was to compare the MagNA Pure 96 Pathogen Universal protocol to the MagNA Pure LC DNA Isolation Kit III that is well established in our microbiological laboratory. For this we isolated nucleic acids from ten different sample materials: body fluids (CSF, urine, sputum), feces, swabs, as well as whole blood, EDTA-, Citrate-plasma and serum. This spectrum is typical for a microbiology laboratory and includes easy and difficult-to-process samples, as sputum and stool are. Into these sample types we spiked classifi ed pathogen stocks from in-house cultures. Subsequently real-time PCR analysis of fungal, bacterial and viral targets was done using the LightCycler® 480 Instrument.

(["Article"], PDF, 803 KB) Download

Performance Efficient Extraction of Viral DNA and RNA from a Variety of Human Sample Materials

Using a Single Standardized Protocol with the MagNA Pure 96 System

(["Article"], PDF, 1042 KB) Download
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