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Total Search results for "SYBR Green" ()

To order these products, please contact your Roche order management team.

Products (17)

LightCycler® 480 SYBR Green I Master

Ready-to-use hot start reaction mix for SYBR Green I-based real-time PCR using the LightCycler® 480 Instrument.

For life science research only. Not for use in diagnostic procedures.

LightCycler® 96 DNA Green Value Pack S

Special bundle combipack, including multiwell plates and real-time PCR master for SYBR Green I assays performed on the LightCycler® 96 Instrument.

For life science research only. Not for use in diagnostic procedures.

LightCycler® 96 DNA Green Value Pack M

Special bundle combipack, including multiwell plates and real-time PCR master for SYBR Green I assays performed on the LightCycler® 96 Instrument.

For life science research only. Not for use in diagnostic procedures.

LightCycler® Nano DNA Green Value Pack L

Special bundle combipack, including reaction device, reaction vessels and real-time PCR master for SYBR Green I using LightCycler® Nano Instruments.

For life science research only. Not for use in diagnostic procedures.

LightCycler® Nano DNA Green Value Pack S

Special bundle combipack, including reaction device, reaction vessels and real-time PCR master mix for SYBR Green I using LightCycler® Nano Instruments.

For life science research only. Not for use in diagnostic procedures.

Publications (62)

A Broadly Reactive One-Step SYBR Green I Real-Time RT-PCR Assay for Rapid Detection of Murine Norovirus 

Keywords: High Pure Viral RNA Kit ,LightCycler Nano ,SYBR green

K. Hanakim F. Ike , A. Kajita , W. Yasuno , M. Yanagiba , M. Goto , K. Sakai , Y. Ami and S. Kyuwa

PLOSone

A real time PCR assay for the detection and quantitation of Mycobacterium avium subsp. paratuberculosis using SYBR Green and the Light Cycler 

Keywords: FastStart Essential SYBR Green master mix ,LightCycler system

J. O'Mahony and C. Hill

J Microbiol Methods, 51, 3, 283 - 293

Simultaneous quantification and genotyping of hepatitis C virus RNA by a two-step Real-time PCR assay on the lightcycler instrument 

Keywords: LightCycler Carousel ,SYBR green

M. Abe , C. Klett , E. Wieland , S. Gille and O. Landt

Folia Med (Plovdiv), 52, 3, 21-30

Gene expression during the induction, maintenance, and release of dormancy in apical buds of poplar 

Keywords: Lightcycler 480 ,Lightcycler 480 SYBR Green I Master Mix ,SYBR Green

A. Rohde , T. Ruttink , V. Hostyn , L. Sterck , K. Driessche , W. Boerjan

J Exp Bio, 58, 15-16, 4047 - 4060

Assessment Techniques to Detect Aspergillus fumigatus in Different Samples of Immunosuppressed Male Western Albino Rats 

Keywords: LightCycler Master Mix ,LightCycler Fast start DNA master SYBR Green Kit

K. Niazi , J.M. Khaled , S.A. Kandeal , A.S. Khalel

Jundishapur Journal of Microbiology,7,11,e11974

Documents (8)

Comparison of qPCR Assays Using SYBR Green I Intercalation or the Universal ProbeLibrary as Detection Format

In our efforts to measure the expression levels of the thyroid hormone receptor ß2 in small areas of mouse brain, we encountered primer-dimer problems when using SYBR Green I assays. With the Universal ProbeLibrary and the LightCycler® 2.0 Instrument we were able to solve the primerdimer problem, increase PCR efficiency, and continue our investigations

(Article, PDF, 296 KB) Download

Improved Performance of the LightCycler® FastStart DNA MasterPLUS SYBR Green I Kit in Highly Sensitive Transcript Quantification

Real-time reverse transcription-PCR (RT-PCR) is considered the method of choice for any experiments requiring sensitive, specific, and reproducible quantification of mRNA molecules of interest. It is an essential tool for basic research, molecular medicine, and biotechnology.

(Article, PDF, 291 KB) Download

Quantitative PCR by Continuous Fluorescence Monitoring of a Double Strand DNA-Specific Binding Dye

A simple method for quantitative PCR using the LightCyclerTM and the dsDNA binding dye SYBR® Green I Dye is demonstrated. The method has a dynamic range of at least six orders of magnitude and a sensitivity of a single copy per reaction. Two methods for removing the confounding signal from nonspecific amplification products are described. The method is suitable for both DNA and RNA quantification.

(Article, PDF, 501 KB) Download

The Universal ProbeLibrary – a Versatile Tool for Quantitative Expression Analysis in the Zebrafish

Here, we describe the comparison of the Universal ProbeLibrary-based qPCR to a conventional SYBR Green-I-based qPCR assay using embryonic zebrafish cDNA.

(Article, PDF, 136 KB) Download

Quantitative RT-PCR: Comparing Real-Time LightCycler Technology with Quantitative Competitive RT-PCR

(Article, PDF, 208 KB) Download
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