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Comparison of qPCR Assays Using SYBR Green I Intercalation or the Universal ProbeLibrary as Detection Format

In our efforts to measure the expression levels of the thyroid hormone receptor ß2 in small areas of mouse brain, we encountered primer-dimer problems when using SYBR Green I assays. With the Universal ProbeLibrary and the LightCycler® 2.0 Instrument we were able to solve the primerdimer problem, increase PCR efficiency, and continue our investigations

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