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Request a Quote for LightCycler® 480 Instrument II
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LightCycler® 480 Instrument II
Rapid high-throughput, plate-based real-time PCR amplification and detection instrument.
For life science research only. Not for use in diagnostic procedures.
|Product No.||Pack Size||List Price||Quantity|
|05015278001||1 instrument 96-well version||
|05015243001||1 instrument 384-well version||
The LightCycler® 480 System is a high-performance, medium- to high-throughput PCR platform (96- or 384-well plates) that provides various methods for gene detection, gene expression analysis, genetic variation analysis, and array data validation. The system features the LightCycler® 480 Instrument, a versatile, plate-based real-time PCR device that supports mono- or multicolor applications, as well as multiplex protocols. The benchtop instrument is easily customizable to meet changing user requirements, and can be integrated into everyday use as a robotically controlled, automated high-throughput solution. The LightCycler® 480 Instrument is designed for general laboratory use and is not intended for use in diagnostic procedures.
- Obtain fast, highly sensitive, and reproducible results for qualitative and quantitative detection of nucleic acids, mutation scanning, and SNP analysis. Use basic and advanced methods for gene expression and genotyping based on endpoint analysis or melting curves.
- Benefit from cutting-edge thermal block and optical technology that leads to excellent well-to-well homogeneity and maximized detection sensitivity.
- Use a wide range of probe formats (e.g., FRET or HybProbe probes, SimpleProbe probes, hydrolysis probes) and fluorescent dyes (e.g., SYBR Green I, ResoLight Dye, FAM, HEX, Cy5, LightCycler® Red 610, LightCycler® Red 640, and LightCycler® CYAN 500).
- Choose the optimal type of multiwell plate (white or clear) to best fit your assay format, application, and workflow. Use the available adapter plate to also work with LightCycler® tube strips.
- Build a modular real-time PCR platform to meet your particular needs. Flexibly switch between plate formats and thermal block cyclers in just a few minutes with no recalibration time, and benefit from additional user-friendly software modules.
- Extract meaningful results via the state-of-the-art data analysis methods, in line with MIQE guidelines. Analyze true raw data without the need for passive reference dyes or normalization plates.
- Protect your data using a system compatible with 21 CFR part 11 requirements.
- Integrate the LightCycler® 480 System as part of a LIMS in combination with robotic pipetting and plate loading.
- LightCycler® 480 Instrument, 96- or 384-well
- LightCycler® 480 Block Cycler Unit, 96- or 384-well
- LightCycler® 480 Software*
- LightCycler® 480 Instrument Operator’s Manual
- LightCycler® 480 LED Lamp (Spare Lamp)
- LightCycler® 480 Multiwell Plates, 96- or 384-well, white
- Pentium Desktop PC
** including absolute quantification, Tm calling, relative quantification, melting-curve-based genotyping, and endpoint genotyping
Use the LightCycler® 480 System to perform:
- Gene detection
- Absolute quantification
- Relative quantification/gene expression analysis
Genetic Variation Analysis
- Melt-curve genotyping with labeled probes
- Endpoint genotyping
- High-Resolution Melting (HRM)
- Multiple Plate Analysis
The LightCycler® 480 Instrument shows outstanding performance in terms of:
- High-performance hardware
- Innovative software
- Specially designed disposables
- Application-optimized reagents for a variety of experiments
Click here to learn more about the LightCycler® 480 System Performance.
Based on a comprehensive improvement of the Peltier-based technology, using heat-equalizing Therma-Base Technology incorporated into a silver block, the LightCycler® 480 Instrument provides extraordinary well-to-well temperature homogeneity and maximized inter-well, inter-cycle reproducibility. The special arrangement of optical components ensures the uniform collection of signals across the plate and makes analysis independent of the sample position on the plate. Channels and filters can be combined in different ways to allow flexibility in monocolor as well as multiplex assays.
|Dimensions (without computer)||
60 cm x 60 cm x 54.5 cm (24 in x 24 in x 21.5 in)
(W x D x H)
|Weight||Approximately 55 kg (121 lbs)|
|Sample Number||96 or 384|
|Reaction Vessel||96-well PCR plate, 384-well PCR plate|
|Reaction Volume||96-well PCR plate: 10 - 100 μl; 384-well PCR plate: 5 - 20 μl|
|Excitation Source||Broad-spectrum, high-intensity LED (390 – 710 nm)|
|Excitation Filters||440, 465, 498, 533, 618 nm (System II)|
|Detection Filters||488, 510, 580, 610,640, 660 nm (System II)|
|Measuring Mode||Real-time, online (continuous display of readings)|
|Temperature Control||Peltier-based heating/cooling|
|Run Time||<40 minutes (384-well PCR plate)|
For more information about the LightCycler® 480 Instrument Technology, click here.
The LightCycler® 480 Real-Time PCR System can be used with:
- Universal ProbeLibrary
- SYBR Green I
- HybProbe Probes
- Hydrolysis Probes
- SimpleProbe Probes
For detailed information, visit Assay Formats for Use in Real-Time PCR.
Due to its highly flexible selection of excitation and emission wavelengths, the LightCycler® 480 Real-Time PCR System is compatible with most currently available dyes and assay formats, yet is also adaptable to new and future applications.
A summary of LightCycler® 480 Real-Time PCR System detection formats is provided in the table below.
The LightCycler® 480 Real-Time PCR System consists of a variety of components that ensure maximum accuracy, versatility, and speed in quantitative real-time PCR and genotyping.
LightCycler® 480 Block Kits
The LightCycler® 480 Block Kits consist of a block cycler unit for 96- or 384-well PCR plates, a corresponding block lid, and a storage box for both accessories. The user can quickly and easily exchange the thermal block cycler unit and the block lid. The new thermal block cycler unit and block lid are automatically detected and calibrated by the LightCycler® 480 Instrument after the instrument is turned on. No additional time-consuming calibrations are necessary.
LightCycler® 480 Software
The LightCycler® 480 Software uses unique Roche algorithms for highly accurate and robust automated data analysis. It enables users to quickly customize the system to meet different application requirements, from basic manual analysis, a wide choice of flexible quantification methods in line with MIQE guidelines, to tools for multiple plate analysis.
LightCycler® 480 Disposables
The LightCycler® 480 Multiwell Plates have been adapted to the special rapid cycling environment of the LightCycler® Thermal Block. LightCycler® 480 Multiwell Plates are available in clear or white. White plates provide maximized sensitivity, especially with HybProbe Probe-based assays. For assays using hydrolysis probes or SYBR Green, clear plates provide an alternative for easier monitoring of PCR setup.
LightCycler® 480 Reagents
The LightCycler® 480 Real-Time PCR System includes generic master mixes tailor-made for each key application in genomics research (gene identification, gene quantification, variation screening, and genotyping).
Click here to compare the LightCycler® 480 Reagents.
The Universal ProbeLibrary (UPL) combines the flexibility, availability, and convenience of SYBR Green I assays with the specificity of hydrolysis probe assays. The unique combination of online assay design software and only 165 pre-tested, real-time PCR probes allows quantification of virtually any transcript in the transcriptome of a large number of organisms.
Get Instructions for Use (IFU) and Material Safety Data Sheets by clicking onto the following links
LightCycler® 480 System Technology
Learn the benefits of this silver thermal block cycler for high level temperature homogeneity.
Use of MagNA Pure 96 and LightCycler® Systems for High-Throughput Testing of Nail Samples for Dermatophytes Detection
Dermatophytes are the major cause of onychomycosis infection of the nail. Conventional laboratory diagnosis is labor intensive, requiring long incubation times and showing poor sensitivity. For routine detection of dermatophytes in nail samples from licensed pedicures, a faster and more accurate detection method is necessary. Molecular testing by PCR has shown in literature to increase sensitivity significantly compared to conventional culture and microscopy methods.
Multiple Plate Gene Expression Analysis with the LightCycler® 480 Real-Time PCR System
Expression Analysis of Genes Involved in Neurodegenerative Disorders
Microsatellite Analysis of Grapevine Varieties by High-Resolution Amplicon Melting Analysis
The speed, resolution, and closed-tube nature of high-resolution melting provide similar benefits as with other real-time PCR applications.
Versatile RNA isolation tools for formalin-fixed paraffin-embedded tissue sections
Gene Expression Analysis You Can Count On
LightCycler® 480 Real-Time PCR System: Innovative Solutions for Relative Quantification
The LightCycler® 480 System includes sophisticated software algorithms that generate data for absolute and relative quantification.
New Application for the LightCycler® 480 System: qPCR-based microRNA-Profiling
miRNA expression quantification as performed here on the LightCycler® 480 System is by far the most sensitive method available, superior over all array-based miRNA profiling systems.
RealTime ready RT-qPCR Assay Development and Qualification
In this document, we describe the processes involved in on-demand RealTime ready RT-qPCR assay development for human, mouse, and rat target genes, and provide an overview on key parameters of more than 10,000 RealTime ready RT-qPCR assays.
The LightCycler® 480 System - Unleash the Potential of Real-Time PCR
Amplify your success in medium- and high-throughput real-time PCR applications with the renowned LightCycler® 480 System from Roche Applied Science.
Utility of the MagNA Pure 96 and LightCycler® 480 Systems for Testing of Stool Samples for Toxin-producing Clostridium difficile
Clostridium difficile infection (CDI) is an important cause of hospital diarrhea. Conventional CDI testing by enzyme immunoassays is fast yet shows poor sensitivity and specificity, whereas the ‘gold standard’ toxigenic culture is laborious and time consuming. Molecular tests for CDI are fast and highly sensitive and specific. Our laboratory has developed a molecular procedure that allows for rapid detection of CDI directly in stool samples. It is based on the isolation of highly pure DNA extracts from feces using the MagNA Pure 96 System, followed by detection of the C. difficile toxins tcdA and tcdB using real-time PCR on the LightCycler® 480 Instrument.
Utility of the MagNA Pure 96 and LightCycler® 480 System for the Detection of Respiratory Pathogens
Acute respiratory disease (ARD) is a major cause of morbidity in developed countries and accounts for a large number of hospitalizations of especially young children. In addition, in developing countries, it constitutes an important cause of death. Viruses were shown to be a major cause of ARD. The last decade has seen the growing importance of molecular techniques in not only the discovery of novel viral respiratory pathogens, but also in routine testing of viral respiratory disease, replacing traditional virus detection methods in many laboratories. Also in our laboratory, a multiplex real-time PCR respiratory panel was introduced a number of years ago, leading to faster analysis and detection. In 2010, the MagNA Pure 96 System was introduced into the laboratory, where it is combined with the LightCycler® 480 Instrument to yield an easy-touse, efficient set-up for molecular research. This paper describes our results obtained with this setup in analysis of viral respiratory samples in 2010 and 2011.
LightCycler® 480 System - High Resolution Melting and Gene Scanning
Learn how to use the High Resolution Melting Master PCR Reagent and Gene Scanning Analysis software of the LightCycler® 480 System using multiwell plates.
LightCycler® 480 System Reagents
Describes the LightCycler® 480 Real-Time PCR System, including master mixes for each application (gene identification, gene quantification, variation screening, and genotyping).
LightCycler® 480 System - Multiple Plate Analysis
Learn how to use LightCycler® 480 Multiple Plate Analysis Software to produce and handle large data sets.
LightCycler® 480 Instrument: High-throughput Genotyping for TLR-8 SNPs
In this study, the LightCycler® 480 Instrument was evaluated for genotyping at high throughputs, based on melting temperature differences observed when different alleles are recognized with HybProbe probes.
LightCycler® 480 System: High-throughput Gene Expression and Genotyping Analysis - A Performance Study
The study demonstrated that the LightCycler® 480 System is a highly versatile and reliable platform for rapid gene expression and genotyping analysis offering enhanced throughputs and automation options for streamlined workflows.
Genotypes in Smokers: Correlation with Smoking Behavior
In order to investigate the feasibility of future genetic analyses, we genotyped 14 SNPs in 288 samples of addicted smokers using the LightCycler® 480 System with HybProbe assays.
Real-Time Multiplex PCR of five Different DNA Targets Using the LightCycler® 480 System
The results show that the LightCycler® 480 Instrument is a convenient and reliable platform for (quantitative) multiplex PCR detection.
Performance Efficient Extraction of Viral DNA and RNA from a Variety of Human Sample Materials
using a Single Standardized Protocol with the MagNA Pure 96 System
Rapid High-Throughput Methylation Analysis Using the LightCycler® 480 System
We assessed the performance of the instrument combined with the LightCycler® 480 High Resolution Melting Master mix for DNA methylation analysis using the methylation-sensitive high resolution melting (MS-HRM) methodology.
Relative Quantification of Multiple mRNA Targets and Reference Genes in Spinocerebellar Ataxia
The LightCycler® 480 System in combination with the Transcriptor First Strand cDNA Synthesis Kit and Universal ProbeLibrary assays, provides a straightforward and productive workflow for processing large numbers of samples for relative quantification using high-throughput real-time PCR.
Comparison of Automated MagNA Pure 96 and Manual PAXgene RNA Isolation from Stabilized Peripheral Blood Samples
Experiments in this paper investigate the quality and capacity of RNA isolation from PAXgene tubes on the MagNA Pure 96 System compared to conventional manual extraction methods. Researchers used a modified RNA isolation protocol (described in the MagNA Pure 96 Cellular RNA Large Volume Kit product insert) that extracts up to 48 stabilized blood samples (or 96 aliquots) simultaneously. Attributes such as purity, recovery, RNA integrity (RIN) and sensitivity in downstream RT-PCR applications were compared and analyzed. The goal for these studies is to develop an optimized and more efficient workflow for the detection of CRC biomarkers in peripheral blood samples.
The E-Method: a highly accurate technique for gene-expression analysis
The E-Method from Roche Applied Science can produce more accurate relative quantification data by compensating for differences in target and reference-gene amplification efficiency.
High-Throughput and Complex Gene Expression Validation Using the Universal ProbeLibrary and the LightCycler® 480 System
In this article, we discuss how the Universal ProbeLibrary and the LightCycler® 480 System provided flexibility in high-throughput confirmatory qPCR analysis of datasets generated from SAGE and microarray platforms in three complex studies.
Quantitative Detection of Legionella pneumophila in Water Samples: Assay Transfer to the LightCycler® 480 Real-Time PCR System
This study investigates how easily the existing Legionella DNA quantification assay could be transferred from the capillary to the plate-based system and which points in the procedure require special consideration.
Identification of Valid Endogenous References for Monitoring Gene Expression Changes in Human Multipotent Mesenchymal Stromal Cells using RealTime ready Assays
Using the RealTime ready Human Reference Gene Panel in conjunction with the RealTime ready Data Import Wizard from GenEx software provides a very fast and pragmatic approach to identify suitable reference genes, resulting in sound scientific data.
LightCycler® 480 Detection Formats
Learn how to adapt the LightCycler® 480 Real-Time PCR System to your applications.
Genotyping using the LightCycler® 480 System
Learn more about melting curve and endpoint analysis using the LightCycler® 480 System.
Expression Analysis of ABC Transporters in Lung Cancer Cell Lines Using a RealTime ready qPCR Focus Panel
The RealTime ready ABC Transporter Panel is an easy-to-use, rapid system for expression profiling of ABC transporter genes.
Rapid and Reliable Methylation Detection in Archival Tissue Samples using High-Resolution Melting Analysis
The aim of the present study was to establish and evaluate HRM assays for detection of promoter methylation on archival FFPE tissues from individuals with colorectal cancer.
LightCycler® 480 Real-Time PCR System in Human Genetics
High-Resolution Melting for CFTR Mutation Scanning
Analysis of the DNA Methylation Patterns at the BRCA1 CpG Island
Bisulfite modification of genomic DNA and PCR is economical and does not require specialized equipment.
Microsatellite Copy Number Detection with the LightCycler® 480 Real-Time PCR System
Adaptation of Smart Amplification Process, version 2 (SMAP-2) assays for microsatellite copy number polymorphism detection for use on the LightCycler® 480 Instrument
qPCR Identification of Genes Involved in Apoptosis and Cell Cycle Regulation
These experiments demonstrate that the RealTime ready Focus Panels are efficient tools for the quantitative analysis of gene expression for genes related to cell cycle and apoptosis as well as for housekeeping genes.
Transfering PCRs to HRM-assays on the LightCycler® 480 System- Examples for BRCA1
To evaluate how easy it is to design hrMCA assays using the LightCycler® 480 Instrument, we selected 3 different gene fragments, designed an MCA assay, and tested its sensitivity to detect known variants.
LightCycler® 480 and 1536 Real-Time PCR Systems - Powerful Solutions for Different Throughput Levels
This cross-platform study represents the sensitivity, reliability, and homogeneity provided across the different throughput formats of the plate-based LightCycler® Systems.
First Steps in Relative Quantification Analysis of Multi-Plate Gene Expression Experiments
The easy initial setup as well as the “Result Export” procedure of the LightCycler® 480 Software is described.
Increased Throughput and Decreased Turnaround Time of enterohemorrhagic E. coli (EHEC) Extraction and Detection in Stool Samples through Use of MagNA Pure 96 System and LightCycler®* Instrument
To detect the EHEC bacterium, the automated MagNA Pure 96 System for nucleic acid isolation and LightCycler®* Real-Time PCR Instrument (Roche Diagnostics) were used for high throughput examination of EHEC directly in stool samples.
Step-by-Step Workflow for Small Sample Quantitative Real-Time PCR Confirmation of Microarray Results
A workflow for small sample quantitative real-time PCR analysis, which includes tissue lysis, RNA isolation, cDNA synthesis and amplification, coupled with the LightCycler® 480 System and data analysis
Mutation Scanning Using High Resolution Melting or dHPLC: a Performance Comparison Study
Very little effort is required to replace dHPLC Systems with the LightCycler® 480 System for mutation scanning and detection in biomedical research and testing.
Genotyping using digital PCR with the LightCycler® 1536 Real-Time PCR System to detect the IDH1 mutationin plasma samples
Findings presented in this report show that combining two qPCR approaches can be useful for detecting mutant tumor DNA in plasma sample material.
LightCycler® 480 System Performance Data
Learn how to increase sensitivity and reproducibility when using the LightCycler® 480 System.
Gene Quantification on the LightCycler® 480 System
Learn how the LightCycler® 480 Software 1.5 offers several methods for precise quantification, and differently determining PCR efficiency.
Relative Quantification with the LightCycler® 480 Real-Time PCR System
Evaluation of RNA stabilization treatments prior to RNA isolation from lung biopsies
Quantitative Chromatin Immunoprecipitation Using the LightCycler® 480 System
The LightCycler® 480 System allows us to do quantitative, multiple ChIP analyses in a time- and cost-effective manner.
Fast qRT-PCR with LightCycler® 480 RNA Master Hydrolysis Probes
This kit is an easy-to-use hot-start reaction mix, specifically adapted for one-step RT-PCR under the rapid and accurate cycling conditions of the LightCycler® 480 Instrument using hydrolysis probes as detection format.
Relative Quantification of miRNA Target mRNAs by Real-Time qPCR
This workflow of easy-to-establish procedures for gene expression analysis is sensitive enough to detect the subtle changes in mRNA levels typically induced by miRNAs.
High Resolution Melting: Optimization Strategies
This technical note describes general steps of setting up HRM-based PCR assays, with a special focus on ways how to optimize procedures for gene scanning experiments
Endpoint Genotyping with the LightCycler® 480 Real-Time PCR System
Association of cannabinoid receptor variants with adiposity in obese men
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