Innovative. Intuitive. Accurate.

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Intuitive for all levels of experience.

Ideal for any level of user, the LightCycler® 96 System offers an intuitive, user-friendly interface and cutting-edge fiber optics. Choose this compact, yet comprehensive system to obtain the accurate and reproducible data you need for publication-ready results and today’s challenging research demands.

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LightCycler® 96 System

An ideal combination of accuracy, temperature homogeneity, and reproducibility.

Innovative

Maximize Well-to-Well Consistency through Innovative Optics
1. Equally excite all 96 wells.

Equal excitation of all wells—particularly in wells at the plate edges—is difficult to achieve in a rectangular plate excited by a round light source. Some suppliers’ systems scan a lower-intensity LED across the plate to excite all wells. Others rely on master mixes containing a passive reference dye that attempts to compensate for these “edge effects.”

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The Roche Solution.

Equally excite all wells at the same time through the system’s high-intensity LED, which simultaneously transmits equal amount of light through the first set of 96 optical fibers.

2. Simultaneously capture the signal from 96 wells.

Simultaneous capture of signals from all 96 wells is equally essential to maximize well-to-well consistency and avoid sample bias. Some suppliers’ systems rely on a scanning head that reads the 96 wells sequentially, not simultaneously, introducing a source of well-to-well inconsistency because of the time lag in data acquisition.

The Roche Solution.

Simultaneously capture the signal of all 96 wells through the second set of 96 optical fibers, each of which is positioned directly over its own well.

3. Maintain temperature homogeneity across the plate.

Temperature inconsistencies across the plate can produce differences in cycling temperatures or “hot spots,” and thus disparate results, in different wells.

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The Roche Solution.

Increase consistency through the LightCycler® 96 System’s silver thermal block cycler (driven by 6 Peltier elements), low mass electro-formed silver mount, and heated lid.

Intuitive

Start your research today with an Intuitive Software Interface
Easily direct the instrument through the state-of-the-art touchscreen
Perform powerful analyses with ease

See a video demonstration of the software.

Accurate

Generate Data You Can Trust
Insist on a high-performance system that generates accurate, publication-ready results you can trust. Take full advantage of the LightCycler® 96 System’s innovative optics and highly homogeneous thermal cycling conditions (Figure A) to produce the precise, reproducible data you need (Figure B) to achieve your research and personal goals.
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Figure A. The LightCycler® 96 System generates reproducible, efficient, and consistent results. The Parvo B19 gene was amplified in 11 serial 1:2 dilutions of plasmid DNA (4 replicates each), from 500,000 copies to 244 copies, and detected with a hydrolysis probe assay. Results show a clear separation of the different dilutions, demonstrating that the system can efficiently discriminate between 2-fold differences, even down to 244 copies. Also, the very close Cq values (ΔCq < 0.2) of the replicates of a given dilution provide evidence for the reproducibility of the LightCycler® 96 System.

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Figure B. Homogeneous and uniform temperature across the block. Real-time PCR was performed using 3 pg of human DNA in each of the 96 block positions. (a) A 71-bp amplicon of the beta-globingene was amplified using hydrolysis probe detection. (b) The reaction product was also subjected to melting curve analysis. Low variation Cq values (Cq range =0.33 and SD=0.06) and overlapping melt curves of the amplicon (Tm range of 0.31°C and SD=0.31) in each of the 96 positions demonstrate temperature homogeneity and equal treatment of all samples - independent of block position.

Stay connected to your results

The LightCycler® 96 system enables you to:

Customer Testimonials

True Well-to-Well Consistency

Brad Smith, Assistant Professor
BYU Hawaii

"We performed a comparison between the StepOnePlus, the CFX, our older IQ5, and our new LightCycler® 96 Instrument. In terms of well-to-well consistency, the LightCycler® 96 clearly stood out among the four instruments. Briefly, we loaded the same sybr green assay in 40 wells of a qPCR plate, distributed among inner and outer wells. We then averaged the Cq values for all wells, and calculated the standard deviation. The chart shows the standard deviation as a percentage of the average Cq value. Of all the instruments, the LC96 had the least variance overall, and was the only instrument that showed no edge effect."

Sean Nygaard, Lab Manager
Grompe Lab
Oregon Health & Science University

well-to-well consistency

Data on file. Data was unsolicited by Roche and generated at the customer site. These were sybr assays, 20 ul per reaction, using 50 ng plasmid template.  For each instrument, the plates, seals, and master mixes were those recommended by the manufacturer. Sean Nygaard received compensation for sharing his data with Roche Diagnostics.

"I work in the laboratory of Prof. Peter Vogt at The Scripps Research Institute, studying the Myc-Max transcription factor. To investigate these sorts of protein-protein interactions, I use the LightCycler® 96 instrument. The LightCycler® 96 instrument has a high sensitivity and allows us to perform our assays in a rapid manner."

Jonathan Hart Research Associate
The Scripps Research Institute

"The LightCycler®96 software has been intuitive and very easy to use. The instrument has helped me generate high quality relative and absolute quantification data, allowing me to discover new targets that are important for my research in pulmonary hypertension."

Harry Karmouty-Quintana, PhD Assistant Professor
Department of Biochemistry and Molecular Biology
The University of Texas Health Science Center at Houston

LightCycler® 96 Details

The LightCycler® 96 System is an optimal mid-size, mid-throughput qPCR solution for academic research, providing an ideal combination of temperature homogeneity and data reproducibility. Comprehensive programming options, a large touchscreen, and intuitive analysis software that is accessible to any user make it easy to obtain publication-ready results. Includes gradient function and options to work with PCR strips or plates.

General Specifications
Dimensions 40 cm x 40 cm x 53 cm
16 in x 16 in x 21 in
(W x D x H)
Weight approx. 27 kg / 55 lbs
Noise level during run 43 dB(A)
Electrical approvals CE, ICE, UL
Reaction volumes 10–50 μl
Sample format 96-well plates, 8-tube strips
Runtime < 40 min for 3-step, 40 cycles PCR, < 1,5 h for HRM
Hardware Specifications
Thermal cycling system Peltier-based, 96-well block
Max ramp rate heating 4.4°C
Average ramp ratecooling 2.2°C
Programmable temperature range 37-98°C
Temperature accuracy ±0.2°C of programmed target temperature
Tm uniformity Range (max-min) 0.4°C; STD < 0.1°C
Gradient function Yes
Gradient operational range 37-98°C
Programmable span (X-Y °C) Max. 20°C gradient range
Excitation High-power broad spectrum LED
Measurement (Integration time) Simultaneous data acquisition for all positions in 10-1000 ms (dynamic mode)
Detection CCD Camera
Optical system Fixed fiber optics with four excitation and four emission filters
No scanning technologies
Cq uniformity Range (max-min) 0.8°C; STD < 0.2°C (enabling resolution of 2-fold concentration differences)

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