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The DIG System

 

Specifically Label and Detect Nucleic Acids 
 

Publishable results require high level specific detection and low background. Do your hybridizations have nonspecific signals and high background?

The DIG System is ideal for nucleic acid labeling. Flexibly use colorimetric, luminescent or fluorescent signal detection. Achieve high sensitivity and low background in very short exposure times
 

  • Specificity: DIG antibodies do not bind other substrates.
  • Versatility: Use DIG labeled probes for filters and in situ hybridization.
  • Proven: Thousands of publications show why DIG is superior to radioactivity.


High specificity and sensitivity are the reasons researchers worldwide choose the DIG System to detect nucleic acids using filter and in situ hybridization. The DIG-labeling method is based on a steroid isolated from digitalis plants (Digitalis purpurea and Digitalis lanata). As the blossoms and the leaves of these plants are the only natural source of digoxigenin, the anti-DIG antibodies only bind to DIG, not other biological substrates.



Use robust procedures and established protocols for straightforward, sensitive, and efficient nonradioactive labeling and detection.




Hybridized DIG-labeled probes may be detected with high affinity anti-digoxigenin (anti-DIG) antibodies that are coupled to either alkaline phosphatase (AP), horseradish peroxidase (POD), or fluorescein and rhodamine for colorimetric, chemiluminescent, or fluorescent detection.
 

For more information, see the following brochures:
 > The DIG System
 > DIG System for In Situ Hybridization
 > DIG System for Filter Hybridization


Detailed technical information can be found in the DIG Manuals:

 > DIG Application Manual for Filter Hybridization
 > DIG Application Manual for Nonradioactive In Situ Hybridization, 4th edition


Learn how to handle the DIG System in our Technical Tips:

 > DIG System Sensitivity and Specificity
 > RNA Labeling using In Vitro Transcription

 

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