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High-performance real-time PCR.
More than a decade of continuous qPCR innovation with the LightCycler® Systems.
Benefit from Roche experience in real-time PCR and achieve precise, consistent results with a full range of instruments, reagents, assays, and disposables to fit any laboratory throughput or budget.
See qPCR in a whole new way.
Generate data you can trust.
Ideal for all levels of experience, the LightCycler® 96 Instrument provides an ideal combination of temperature homogeneity and data reproducibility. Comprehensive programming options, a large touchscreen, and inuitive analysis software that is accessible to any user make it easy to obtain publication-ready results.
Discover which Real-Time PCR System is right for you.
The tools for your research start here.
|LightCycler® 96||LightCycler® 480||LightCycler® 1536||LightCycler® 2.0 *|
|Reactions per Run||96||96⁄384||1536||32|
|PCR Reactions Volume||10 - 50 µl||5-20 µl ⁄ 10- 100 µl||0.5 - 2 µl||20 µl|
|Run Time||< 40 min||<40 min (384) ⁄ <60 min (96)||< 50 min||< 30 min|
|1536-multiwell plates||Glass Capillaries|
|Light Source||High-intensity LED||High-intensity white LED||Xenon lamp||Blue LED|
* LightCycler® 2.0 Instrument conforms to the European directive for in vitro diagnostic medical devices 98/79/EC. For in vitro diagnostic use.
Request a quote for a real-time PCR system.
Optimized reagents for each application.
Easily design your qPCR Assays or function-tested custom qPCR Panels.
Innovative qPCR Instruments.
Generate results you can trust.
Amplify your success in real-time PCR applications with the LightCycler® Instruments. Choose the system that best suits your needs, with flexible sample volumes and throughputs ranging from 32 to 1536 samples per run. All of our instruments provide outstanding temperature uniformity and homogeneity, as well as unbiased data capture, enabling you to quickly generate results that are accurate and reproducible.
LightCycler® 96 System
Intuitive for all levels of experience.
The LightCycler® 96 System is a mid-throughput qPCR solution for academic researcher, providing an ideal combination of temperature homogeneity and data reproducibility. Comprehensive programming options, a large touchscreen, and intuitive analysis software make it easy to obtain publication-ready results.
LightCycler® 480 System
Versatile for experienced users.
96- to 384-well throughput
The LightCycler® 480 System is a high-performance, flexible throughput qPCR platform (interchangeable 96- or 384-well blocks) that provides various methods for gene detection, gene expression analysis, genetic variation analysis, and pathogen detection.
LightCycler® 1536 System
High Throughput - Redefined.
The LightCycler® 1536 Instrument is a rapid, plate-based, thermal block cycler with integrated real-time, online detection capabilities. Take advantage of the first plate-based real-time qPCR instrument that provides 1,536 data points in a single PCR run in less than 50 minutes.
LightCycler® 2.0 System
A proven standard of excellence.
This LightCycler® Carousel-Based System has repeatedly set the standard for accurate and fast real-time PCR. It was the first system to introduce hybridization probes, true melting curve analysis, automated absolute quantification, relative quantification with efficiency correction, and more.
LightCycler® 2.0 Instrument conforms to the European directive for in vitro diagnostic medical devices 98/79/EC. For in vitro diagnostic use.
Designed to maximize the performance of your system.
Rely on Roche proprietary PCR enzymes and formulations to consistently deliver exceptional sensitivity and specificity for your real-time PCR assays.
LightCycler® Multiplex MastersNEW
Made to discover more.
Bring your qPCR to the next level with the new 5x concentrated multiplex master mixes. Detect a greater number of targets per reaction, work more efficiently, and maximize your valuable samples.
For general laboratory use.
EagleTaq Universal Master MixNEW
Change your master mix, not your protocols.
A ready-to-use 2x concentrated master mix suitable for all real-time instruments on which a ROX reference dye is needed for quantitative analysis.
qPCR Reagents for Roche Instruments
|Roche Master Mix||FastStart Essential DNA Probes Master||FastStart Essential DNA Green Master||
SYBR Green I Master
RNA Master Master Hydrolysis Probes
Multiplex RNA Virus Master
Multiplex DNA Master
|Applications||Gene expression||Gene expression||Gene expression||High resolution melting||Multiplex pathogen detection|
|Starting Material||DNA||DNA||RNA||DNA||Viral RNA||Pathogen DNA|
|Chemistry||Hydrolysis probes||SYBR Green I||Hydrolysis probes||SYBR Green I||Hydrolysis probes||ResoLight Dye||Hydrolysis probes|
|Instrument Compatibility||LightCycler® 96||LightCycler® 480||
qPCR Reagents for Non-Roche Instruments
|Roche Master Mix||FastStart Universal Probe Master Mix (ROX)||FastStart Universal SYBR Green Master Mix (ROX)||FastStart TaqMan Probe Master||FastStart SYBR Green Master||EagleTaq Universal Master Mix (ROX)|
|Applications||Gene expression||Gene expression||Gene expression,Genotyping|
|Chemistry||Hydrolysis probes||SYBR Green I||Hydrolysis probes||SYBR Green I||Hydrolysis probes|
|Instrument Compatibility||non-Roche instruments including ViiA7, StepOne Plus, ABI 7500, ABI 7900 HT, Bio-Rad CFX 96 Touch||non-Roche instruments including ViiA7, StepOne Plus, ABI 7500, ABI 7900 HT, Bio-Rad CFX 96 Touch||non-Roche instruments including ViiA7, StepOne Plus, ABI 7500, ABI 7900 HT, Bio-Rad CFX 96 Touch|
Downscale reagent volumes and accelerate the workflow.
Optimal reagents for rapid reactions in capillaries.
For robust and unbiased cDNA synthesis.
Find articles to
Search for genomic workflow publications.
Easily design your qPCR assay to analyze over 5 million transcripts of any sequenced organism.
Function-tested qPCR single assays or custom panels, for human, mouse or rat targets of your choice.
qPCR Assays and Panels
Easy to design. Ready to run.
Choose from a variety of Roche probe-based qPCR assay solutions for your gene expression needs. Opt for universal probes for affordable and easy to design assays. Or select function-tested single assays and customized panels to skip design and optimization steps. Rely on Roche qPCR assay solutions to generate reliable and consistent real-time PCR results,along with complete assay information to facilitate compliance with the MIQE guidelines.
Cost-effective, probe-based assays.
Experience how fast and easy it is to design a specific, cost-effective, hydrolysis probe-based qPCR assay in 3 easy steps using our free online software.
RealTime ready Assays
Function-tested probe-based assays.
Skip time-consuming assay design and optimization steps with the function-tested RealTime ready qPCR assays for gene expression for human, mouse, rat or other species. Receive ready-to-use probe-based assays, compatible with any real-time PCR instrument.
Select Your Assays
RealTime ready Custom Panels
Function-tested qPCR assay Panels.
Create your own gene expression panels with your targets of choice on LightCycler® Plates (96- or 384-well). All assays are function-tested and pre-plated for the upmost convenience and quick time to results.
Roche probe-based qPCR assay solutions
|Assay||Universal ProbeLibrary Single Probes||Universal ProbeLibrary Library||RealTime ready Single Assays||RealTime ready Focus Panels||RealTime ready Custom Panels|
|Format||Single probe (2 vials)||90 most common probes for human targets (90 vials)||Custom Assay, single tube||Pre-configured, pre-plated (96- or 384-well)||Custom, pre-plated (96- or 384-well)|
|Included Components||Probe||90 probes||Probe and Primers||Probes, Primers and Plates||Probes, Primers and Plates|
|Validation||In silico||In silico||Function-tested||Function-tested||Function-tested|
|Assay Information||Complete assay information provided with all Roche qPCR assays|
|Instrument Compatibility||qPCR Instruments detecting FAM||qPCR Instruments detecting FAM||qPCR Instruments detecting FAM||
Working with limited
amounts of RNA?
Learn more about RealTime ready Pre-Amp cDNA Master and Pre-Amp Pirmer pools.
Easily multiplex with your target UPL assay.
Identify the reference genes with the least variability for your test system.
Designed to maximize performance.
LightCycler® Multiwell Plates
Optimal fit and performance
Fully exploit the advanced thermal and optical characteristics of your LightCycler® 96 or 480 System with tailor-made LightCycler® Multiwell Plates to allow optimal heat transfer and optical compatibility.
LightCycler® 8-Tube Strips
Ideal consumable for low throughput.
Use the LightCycler® 8-Tube Strips as plastics disposables that match exactly your LightCycler® 96 or 480 System block geometry for optimal fit and real-time PCR results
Want to simplify plate tracking?
Rely on LightCycler® Multiwell Plates with 4 barcodes for tracking and automation.
LightCycler®1536 Multiwell Plate
Increase your throughput with 1536 data points per run.
Rely on the renowned capillaries to maximize PCR performance.