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cOmplete Products - Protein protection from lysis to purification
> cOmplete Protease Inhibitors
> cOmplete His-Tag Purification
> cOmplete Cell Lysis
> PhosSTOP Phosphastase Inhibition
> Individual Protease Inhibitors
> Protein Processing
> Protein Characterization
Roche protein research techniques build on years of experience in protein labeling, detection, purification and stabilization. cOmplete products protect your proteins during cell lysis, by offering reliable and best-in-class protease inhibition and with full compatibility with EDTA and DTT when performing His-Tag Purification with IMAC.
> cOmplete ULTRA Protease Inhibitor Tablets
cOmplete ULTRA Tablets are the most powerful protease inhibitor cocktail available. In addition to our well known and reliable cOmplete Tablets cOmplete ULTRA Tablets inhibit aspartic proteases for maximum protection.
Please visit the selection table
> cOmplete Protease Inhibitor Tablets
The cOmplete Tablets were the first inhibitor cocktail tablets on the market and invented by Roche. They are available in all varieties (with and without EDTA; blister pack and glass vials) and a safe and well-known solution against protein degradation by proteases. For more than 10 years thousands of researchers have protected their proteins with cOmplete Tablets.
> cOmplete His-Tag Purification Resin and cOmplete His-Tag Purification Columns
The cOmplete His-Tag Purification Resin is a high-capacity IMAC (Immobilized Metal Affinity Chromatography) matrix for convenient single-step purification of His-tagged proteins from lysates. Its nickel-chelate chemistry is truly compatible with EDTA and DTT, and a wide range of buffer substances and salt conditions. The resin is available in loose format or as prepacked columns (1 ml and 5 ml) for use with FPLC systems.
> cOmplete Lysis Kits
cOmplete Lysis Kits make cell lysis easier and faster than traditional methods such as freeze-thaw cycles, sonicators and glass beads. At the same time that cells are lysed, a multitude of proteases can be inhibited. Simply add the cOmplete Lysis Kits optimized for bacteria and mammalian cells.
> PhosSTOP Tablets
PhosSTOP Phosphatase Inhibitor Cocktail Tablets are a proprietary, non-toxic blend of phosphatase inhibitors, which dissolve quickly in aqueous solutions (buffers) and work also in buffers containing formalin for formalin-fixation. PhosSTOP is formulated as a ready-to-use tablet since some of the phosphatase inhibitors are only necessary in nanomol amounts.
Cells contain different types of proteases. Therefore, usually more than one protease inhibitor is needed for complete preservation of the cell's protein(s) which shall be isolated for subsequent experiments, such as 2-D electrophoresis, mass spectrometry, liquid chromatography, specific protein-activity assays, or other experiments performed in proteome research.
Highly purified and specific proteases are essential tools for proteome research. In particular proteins that will be analyzed by mass spectrometry usually must be cleaved after purification (e.g., by 2-D gel electrophoresis). A reproducible cleavage pattern is a prerequisite for clear identification. Trypsin and, to a smaller extent, other endoproteinases (endoproteinases Arg-C, Lys-C, Glu-C) are used to perform these cleavages.
Roche offers a recombinant trypsin, proteomics grade, to specifically address these requirements. In addition, we offer different sequencing-grade endoproteinases suitable for the high-performance cleavages listed below
Following identification of a specific gene product, the protein(s) must be further characterized. For instance, glycan analysis can show whether a protein went through the secretory pathway. For further functional characterization with regard to, for example, subcellular localization or expression patterns in different cell types, labeling and detection tools are required. Analysis of the protein of interest is done either by the generation of a corresponding antibody or by the introduction of an epitope tag, a short amino acid sequence. Furthermore, labeling of purified proteins or antibodies with biotin, digoxigenin, fluorescein, or enzymes, such as peroxidase (POD) or alkaline phosphatase (AP), enables their detection. Finally, methods such as Western blotting or ELISA are used for the qualitative or quantitative detection of certain proteins.
Tools for the detection and characterization of carbohydrate modifications.
Labeling of Proteins and Detection of Proteins
Kits and reagents for the labeling of proteins with ligands, such as alkaline phoshatase, biotin, digoxigenin, fluorescein, or peroxidase, and for the detection of proteins that have been modified with such ligands.
Antibodies for the detection of proteins carrying commonly used epitope sequences like the influenza hemagglutinin protein (HA), human c-myc protein, or six histidine residues (HIS6).
Everything you need for the detection of proteins by chemiluminescence or chromogenic methods following Western blotting.
Kits and reagents for the detection of proteins using the ELISA technique.
Protease Inhibitors Selection Guide
See also the Protein Purification Selection Guides
> Protein Purification via Tag
> Protein Purification via Antibody
Use the following information to select the appropriate protease inhibitor for your application or see the full product list:
|cOmplete ULTRA Tablets, EDTA-free||+++||++||+|
|cOmplete ULTRA Tablets||+++||++||+||+|
|cOmplete Tablets, EDTA-free||++||+|
Individual Protease Inhibitors
|Bestatin||+ (Amino peptidases)|
Pefabloc® SC PLUS
for the inhibition of:
|Antipain Dihydrochloride||Papain, Trypsin (plasmin)|
|Calpain Inhibitor I||Calpain I > Calpain II|
Trypsin Inhibitor (chicken egg white)
Trypsin Inhibitor (soybean)
|Tag||Detection via Western blotting||IMAC||Immuno-precipitation||Immunoaffinity Purification||Immuno-fluorescence|
||Streptavidin-POD||Streptavidin Mutein Matrix||Streptavidin Mutein Matrix. Lowered biotin dissociation constant (1.3x10-7M) allows elution of biotinylated proteins||Avidin-Fluorescein or Avidin-Rhodamine|
||Anti-GFP||Anti-GFP||Anti-GFP||Direct green fluorescence of fused proteins or Anti-GFP with secondary antibody|
Anti-HA Affinity Matrix, Anti-HA High Affinity
Anti-HA Affinity Matrix
|Anti-HA-Biotin, Anti-HA Fluorescein|
|cOmplete His-Tag Purification Resin and Column||
|cOmplete His-tag Purification Resin and Column||Anti-His6 (2)|
||Anti-c-myc, Anti-c-myc POD||Anti-c-myc||Anti-c-myc||Anti-c-myc with secondary antibody|
|Protein C||Anti-Protein C-POD||Anti-Protein C, Anti-Protein C Affinity Matrix||Anti-Protein C, Anti-Protein C Affinity Matrix||Anti-Protein C with secondary antibody|
Use this table to select the appropirate product to purify antibodies from solutions
++= strong binding; += moderate binding; -=no binding
|Organism||Antibody||Protein A||Protein G||Protein L|
|k light chain||-||-||++|
Ultra Efficient Protein Protection
In just minutes, proteases can destroy the proteins you have spent days isolating. cOmplete Tablets were the first inhibitor tablets on the market to defend against proteases. Now you can keep your proteins safer with next-generation cOmplete ULTRA Tablets, which inhibit an even wider range of proteases and offer improved inhibition of serine and cysteine proteases:
cOmplete ULTRA Tablets contain a proprietary blend of powerful protease inhibitors. They are easy to handle: simply add a tablet to your homogenization buffer and instantly protect your proteins against a broad range of proteases, ensuring laboratory safety as you avoid contact with individual hazardous compounds. cOmplete ULTRA Tablets contain both irreversible and reversible protease inhibitors.
Inhibition ratio of cOmplete ULTRA Tablets versus other protease inhibitors in lysate and in individual proteases:
|Figure 1: Inhibition ratio of proteases in CHO K1 lysate by cOmplete ULTRA Tablets with EDTA in relation to other tablets.||Figure 2: Inhibition ratio of Proteinase K by cOmplete ULTRA Tablets containing EDTA in relation to other inhibitor cocktails.|
Combine cOmplete ULTRA tablets with PhosSTOP Tablets for simultaneous protection against proteases and phosphatases.
IMAC purification now truly compatible with EDTA and DTT
|cOmplete His-Tag Purification Resin is an innovative high-capacity IMAC matrix (Immobilized Metal Affinity Chromatography) for convenient single-step purifications of His-tagged proteins from total lysates. Roche's propriety nickel-chelate chemistry ensures extraordinary compatibility with commonly used reducing agents such as DTT, chelating metalloprotease inhibitors such as EDTA, and a wide range of buffer substances and salt conditions. The wide choice of compatible ingredients allows optimization of buffers for maximum protein stability and solubility.|
cOmplete His-Tag Purification Columns are prepacked with cOmplete His-Tag Purification Resin and can be used with purification systems such as ÄKTA Systems by GE Healthcare.
Click here for more product details.
For tips on how to optimally use cOmplete His-tag Purification Products see the Quick Protocol.
cOmplete His-Tag Purification Resin is the only resin for purifying his-tagged proteins without compromises.
Use the buffer conditions best suited to your protein
Keep your protein comfortable and let it, not your purification resin, determine whether you use DTT, EDTA, or other buffer substances.
Repeatedly obtain highly pure protein
Single step purification without resin recharging.
Protect your protein from toxic nickel
Reduce protein oxidation and aggregation caused by resins that leach nickel.
Work in a safe and eco-friendly environment
Avoid handling of toxic nickel and completely eliminate disposal costs
Figure 1: Tight binding of nickel visualized.
Before (left) and after (right) photos of cOmplete His-Tag Purification Resin after 5 times reuse without recharging. The resin remains blue both before and after reusing, indicating that there is little to no nickel leakage.
Protein-binding performance with His6 CFP.
cOmplete His-Tag Purification Resin (blue columns) with 10 mM DTT and EDTA is reused without nickel recharging alongside Resin G (grey columns) with 1 mM EDTA and 5 mM DTT ( as specified in manufactor's package insert). Another competing product, Resin Q (not shown), did not bind any protein at all.
Loss of resin Ni ions under stringent conditions.
One milliliter each of cOmplete His-Tag Purification Resin and 2 commercially available resins were incubated in 9 ml of a buffer containing 50 mM NaH2PO4, 300 mM NaCl, pH 8.0, 10 mM EDTA, 10 mM DTT, and 500 mM imidazole. The cOmplete Resin lost less than 1 percent of nickel ions.
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