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LightCycler

High performance real-time PCR:

More than a decade of continuous qPCR innovation with the LightCycler® Systems.

Roche continues to innovate, and with that comes a dedication to the scientists who use the LightCycler® system for their experimental needs. Benefit from Roche's experience in real-time PCR and achieve precise, consistent results with a full range of instruments, reagents, assays, and disposables to fit any laboratory throughput or budget. 

Choose the LightCycler® System that's right for you.

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Innovative qPCR Instruments.

Generate results you can trust.

Amplify your success in real-time PCR applications with the LightCycler® Instruments. Choose the system that best suits your needs, with flexible sample volumes and throughputs ranging from 32 to 1,536 samples per run. All of our instruments provide outstanding temperature uniformity and homogeneity, as well as unbiased data capture, enabling you to quickly generate results that are accurate and reproducible.

Discover which real-time PCR System is right for you.

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Discover which real-time PCR System is right for you.

The tools for your research start here.

The tools for your research start here.

  LightCycler® 96 System LightCycler® 480 System
Reactions per Run 96 96/384
PCR Reactions Volume 10 - 50 µl 5-20 µl ⁄ 10 - 100 µl
Run Time <40 min <40 min (384) ⁄ <60 min (96)
Disposables 96-multiwell plates
8-tube strips
 96/384-multiwell plates
8-tube strips
Multiplexing Capability 4x 6x
Automation-Capable No Yes
Light Source  High-Intensity LED  High-Intensity white LED
qPCR Reagents

Designed to maximize the performance of your system.

Rely on Roche proprietary PCR enzymes and formulations to consistently deliver exceptional sensitivity and specificity for your real-time PCR assays.
Choose the reagent that's best for your experiments on:

 

qPCR Reagents for Roche Instruments

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qPCR Reagents for Roche Instruments

  FastStart Essential DNA Probes Master FastStart Essential DNA Green Master LightCycler® 480 Probes Master LightCycler® 480 SYBR® Green I Master LightCycler® 480 RNA Master Hydrolysis Probes LightCycler® 480 HRM Master LightCycler® Multiplex RNA Virus Master LightCycler® Multiplex DNA Master
Applications Gene expression Gene expression Gene expression Gene expression Gene expression High resolution melting Multiplex pathogen detection Multiplex pathogen detection
Starting Material DNA DNA DNA DNA RNA DNA Viral RNA Pathogen DNA
Chemistry Hydrolysis probes SYBR® Green I    Hydrolysis probes SYBR® Green I Hydrolysis probes ResoLight Dye Hydrolysis probes Hydrolysis probes
Instrument Capability LightCycler® 96 Instrument LightCycler® 96 Instrument LightCycler® 480 Instrument LightCycler® 480 Instrument LightCycler® 96 & LightCycler® 480 Instruments LightCycler® 96 & LightCycler® 480 Instruments LightCycler® 96 & LightCycler® 480 Instruments LightCycler® 96 & LightCycler® 480 Instruments

qPCR Reagents for Non-Roche Instruments

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qPCR Reagents for Non-Roche Instruments

  FastStart Universal Probe Master Mix (ROX) FastStart Universal SYBR® Green Master Mix (ROX) FastStart TaqMan Probe Master FastStart SYBR® Green Master
Applications Gene expression Gene expression Gene expression Gene expression
Starting Material DNA DNA DNA
 DNA
Chemistry Hydrolysis probes SYBR® Green I    Hydrolysis probes SYBR® Green I
ROX Yes Yes No No
Instrument Capability non-Roche instruments requiring ROX such as ViiA7, StepOne Plus, ABI 7500, ABI 7900 HT. non-Roche instruments requiring ROX such as ViiA7, StepOne Plus, ABI 7500, ABI 7900 HT.
 non-Roche instruments not requiring ROX such as Bio-Rad CFX 96 Touch, RotorGene Q. non-Roche instruments not requiring ROX such as Bio-Rad CFX 96 Touch, RotorGene Q.
LightCycler® Software

To download the LightCycler® Software, please first register by completing this form. You will receive an email with a link for downloading the software installer, demo data files and the user guide pdf.

 

LightCycler® 480 System customers using software version other than 1.5 and upgrading to 1.5.1 please contact your Roche representative.

 

LightCycler® Carousel-Based System customers please note that the LightCycler® QC Test Software 3.0 is for life science research only and not for use in diagnostics procedures. In case you need to qualify IVD LightCycler® Instrument, contact your Roche representative.

Technical documents

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Products are for Life Science Research Use Only, not for use in diagnostic procedures, unless otherwise indicated.

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