Genotyping of SNPs has become a key component of genetic studies. Identify and map SNP markers to study the genetic basis of complex diseases, detect common disease-associated mutations with the final aim to create personalized drugs that are individually tailored for each person’s genetic make-up.
Rely on Roche's Genotyping solutions to support your detection of known variants or discovery of new variants.
For detection of known variants, two different methods of SNP analysis are available:
- The basic, most commonly used me thod is Endpoint Genotyping analysis using enzymatically cleaved hydrolysis probes.
- A more advanced method is Melting Curve Genotyping analysis using hybridizing HybProbe or SimpleProbe probes.
Using the LightCycler® system, perform endpoint genotyping and melting curve genotyping in order to identify known genetic mutations quickly and easily. For unknown mutations, take advantage of HRM analysis to discover single-point mutations on a stretch of DNA, also known as gene scanning. When combined with DNA bisulfite modification protocols, such high resolution melting approaches can also be adapted to the study of methylation patterns.
Roche continues to innovate, and with that comes a dedication to the scientists who use the LightCycler® system for their experimental needs. Benefit from Roche's experience in real-time PCR and achieve precise, consistent results with a full range of instruments, reagents, assays, and disposables to fit any laboratory throughput or budget.