For general laboratory use. Others DNA Process Control Kit GLU DNA Process Control Kit This product is not available in all territories due to different national regulations.This document is not intended for use in the USA. 22.214.171.124.3.3 DNA Process Control Kit 04038377029450 1 kit true DNA Process Control Kit 07339542001 6 x 96 DNA Control reactions and 600 qPCR reactions of 20 μL final volume each Reagents, kits DNA Process Control Kit 04038377029443 1 kit true DNA Process Control Kit Trial Pack 07339666001 2 x 96 DNA Control reactions and 200 qPCR reactions of 20 μL final volume each Reagents, kits DNA Process Control Detection Kit 04038377029436 1 refill kit for the DNA Process Control detection only. true DNA Process Control Detection Kit 07339623001 400 qPCR reactions of 20 μL final volume each. Reagents, kits en The DNA Process Control Kit is a tool to be used to control for potential failures of sample preparation, amplification, detection, and handling errors. The product is intended for use with a variety of sample materials, such as blood, serum, stool, or urine, and a variety of DNA targets.The DNA Process Control Kit is intended for general laboratory use. Any use of the product for in vitro diagnostic tests is the sole responsibility of the operator and must be validated by the operator following the relevant national regulations. en The DNA Process Control Kit contains a nuclease-resistant DNA that can be added at various steps during the nucleic acid purification of DNA detection workflows. Successful detection of this control DNA serves as a positive control, verifying the functionality of the DNA purification as well as the detection using quantitative PCR (qPCR). It also contains the LightCycler® Multiplex DNA Master optimized for multiplex reactions, ensuring performance for up to 6 targets plus the internal process control. en Background informationTwo major outcomes can occur during a typical detection workflow for DNA targets. The sample can be either positive or negative. Whereas a positive detection also verifies the functionality of workflow components, a negative test outcome could be due either to a true negative sample or to a failure of critical workflow components and thus be a false negative. To rule out the possibility of workflow failures, a positive control that is similar to the target material but inert to the target detection is supplied in this kit and can be used in the workflow.In order to prevent any cross-reaction with sample-derived nucleic acids or target-specific detection systems, the DNA Process Control Assay amplicon has been designed to have no significant homologies to any other known sequence. The DNA Process Control Detection Assay primers and probe are added in a low concentration to further lower any possible competition effects in multiplex qPCR amplifications.The DNA Process Control concentrate is formulated to achieve a robust Cq value within one specified workflow. Different sample materials and workflows may require adjusted dilution of the DNA Process Control concentrate.The DNA Process Control Kit is designed for the detection of an endogenous heterologous control (DNA Process Control) during a sample purification procedure when using:– either a MagNA Pure System together with a dedicated reagent kit (for automated isolation),– or a High Pure Nucleic Acid Isolation Kit (for manual isolation).How this product worksThe DNA Process Control Kit provides a non-competitive internal control to monitor nucleic acid purification and detection processes in order to prevent false-negative results. The DNA Process Control working solution contains a constant amount of nuclease-resistant DNA(1) that can be added to a large variety of sample materials without impairing the purification of other sample intrinsic nucleic acid(2), such as from DNA viruses.The strictly monitored production and quality processes in conjunction with the easy-to-use workflow ensure that constant amounts of the control DNA are added to each and every sample.The DNA Process Control has a unique and completely artificial sequence that makes primer competition with any other target parameters less likely. The primer and the probe of the DNA Process Control are designed to only amplify and detect the unique sequence. The Cy5-labeled probe ensures that other target-specific assays with common dyes such as FAM, Yellow 555, or Red 610 can be combined in multiplex setups.Test conceptThe control is added to the sample material and co-purified with all other sample endogenous nucleic acids. After purification, the DNA Process Control as well as any target-specific parameter is detected in a qPCR reaction.In monoplex reactions in single wells, the DNA Process Control must be positive.In multiplex reactions, the DNA Process Control detection must be positive in all target-negative samples. In target-positive samples, the DNA Process Control may be out-competed by the target-specific assay and a negative control result is allowed.Any failure of the DNA Process Control Detection Assay in target-negative samples indicates an erroneous purification/detection workflow and the sample must be retested.The key steps in the process are:DNA Process Control working solution is added to the sample material.The protective coat of the internal control is lysed during the extraction process, thus enabling co-purification of the control DNA with other sample endogenous nucleic acids.The sample eluates are used for qPCR reactions.– The DNA Process Control Detection Assay specifically detects the internal control.– Laboratory-developed assays assess the status of other targets in the sample material.Since the DNA Process Control is added to all samples, its successful detection proves the correct sample processing as well as the functionality of the generic detection reagents.
DNA Process Control Kit
Includes DNA Process Control, Control Assay, and multiplex master mix for monitoring the whole workflow, from nucleic acid extraction to qPCR.