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Vial / Bottle	Cap	Label	Function / Description	Catalog Number	Content
1	red	FastStart Essential DNA Probes Master, 2x conc.	Ready-to-use hot start PCR mix. Contains FastStart Taq DNA polymerase, reaction buffer, dNTP mix (with dUTP instead of dTTP), and MgCl2.	06 402 682 001	5 vials, 1 mL each
06 924 492 001	10 vials, 5 mL each
2	colorless	FastStart Essential DNA Probes Master, Water, PCR Grade	To adjust the final reaction volume.	06 402 682 001	5 vials, 1 mL each
06 924 492 001	2 vials, 25 mL each

", "Language": "en", "Country": "XG", "Code": "Content" }, { "Name": "Principle", "Value": "Sequence-specific detection of PCR products relies on sequence-specific oligonucleotide probes that are coupled to fluorophores. These probes hybridize to their complementary sequence in target PCR products. Hydrolysis probe chemistry uses the so-called FRET principle. Fluorescence Resonance Energy Transfer (FRET) is based on the transfer of energy from one fluorophore (the donor or reporter) to another adjacent fluorophore (the acceptor or quencher).
Hydrolysis probe assays can technically be described as homogeneous 5'-nuclease assays, since a single 3' non-extendable probe, which is cleaved during PCR amplification, is used to detect the accumulation of a specific target DNA sequence. This single probe contains two labels, a fluorescent reporter and a quencher, in close proximity to each other. When the probe is intact, the quencher dye is close enough to the reporter dye to suppress the reporter fluorescent signal (fluorescent quenching takes place via FRET). During PCR, the 5'-nuclease activity of the polymerase cleaves the hydrolysis probe, separating the reporter and quencher. The reporter dye is no longer quenched and emits a fluorescent signal when excited.
The LightCycler^® PRO and the LightCycler^® 96 Instrument are factory calibrated for commonly used reporter dyes as listed in the respective systems. For example, different labeled hydrolysis probes can be used separately or in combination, which permits up to four-color (LightCycler^® 96 Instrument) or up to seven-color (LightCycler^® PRO Instrument) detection. There is no need for color compensation runs.FastStart Essential DNA Probes Master is a ready-to-use reaction mix specifically developed for the hydrolysis probe detection format in:

• LightCycler^® 480 Multiwell Plates 96 or,
• 8-Tube Strips without an adapter plate on the LightCycler^® 96 Instrument or,

• LightCycler® 480 Multiwell Plates 96 or,
• LightCycler® 480 Multiwell Plates 384 or,
• 8-Tube Strips with the LightCycler^® 8-Tube Strip Adapter Plate on the LightCycler^® PRO Instrument.

The master contains FastStart Taq DNA Polymerase for hot start PCR, which significantly improves the specificity and sensitivity of PCR by minimizing the formation of nonspecific amplification products.
FastStart Taq DNA Polymerase is a chemically modified form of thermostable recombinant Taq DNA Polymerase that shows no activity up to +75°C. The enzyme is active only at high temperatures, where primers no longer bind non-specifically. The enzyme is completely activated (by removal of blocking groups) in a single pre-incubation step (+95°C, 5 to 10 minutes) before cycling begins. Activation does not require the extra handling steps typical of other hot start techniques.", "Language": "en", "Country": "XG", "Code": "Principle" }, { "Name": "Applications", "Value": "The FastStart Essential DNA Probes Master is a ready-to-use hot start reaction mix designed for detecting DNA targets with hydrolysis probes. It allows very sensitive detection and quantification of defined DNA sequences as well as endpoint genotyping analysis. The kit may also be used in other types of PCR on the LightCycler^® PRO and LightCycler^® 96 Systems.
The kit can also help prevent carryover contamination during PCR (when used with LightCycler^® Uracil-DNA Glycosylase) or to perform the second step of a two-step RT-PCR.
In principle, the FastStart Essential DNA Probes Master can be used to amplify and detect any DNA or cDNA target. However, the detection protocol must be adapted to the reaction conditions of the LightCycler^® PRO or LightCycler^® 96 Instruments, and specific PCR primers and probes must be designed for each target.", "Language": "en", "Country": "XG", "Code": "Applications" }, { "Name": "Product Description", "Value": "FastStart Essential DNA Probes Master is a ready-to-use reaction mix containing FastStart Taq DNA Polymerase for hot start PCR, which significantly improves the specificity and sensitivity of PCR by minimizing the formation of nonspecific amplification products. The 2x master mix is optimized for a fixed MgCl₂ concentration, which works with nearly all primer combinations.
No adjustment in the MgCl₂ concentration is needed to amplify different sequences; only template DNA, PCR primers, and hydrolysis probes must be added.", "Language": "en", "Country": "XG", "Code": "Product Description" }, { "Name": "Product Characteristics", "Value": "Ready-to-use hot start reaction mix for real-time PCR with the LightCycler^® PRO or the LightCycler^® 96 Systems.", "Language": "en", "Country": "XG", "Code": "Product Characteristics" }, { "Name": "Product Purpose", "Value": "FastStart Essential DNA Probes Master is a ready-to-use reaction mix containing FastStart Taq DNA Polymerase for hot start PCR, which significantly improves the specificity and sensitivity of PCR by minimizing the formation of nonspecific amplification products. The 2x master mix is optimized for a fixed MgCl₂ concentration, which works with nearly all primer combinations.
No adjustment in the MgCl₂ concentration is needed to amplify different sequences; only template DNA, PCR primers, and hydrolysis probes must be added.", "Language": "en", "Country": "XG", "Code": "Product Purpose" } ] } } ] }