{ "ProductData": { "RelatedLinks": "", "RegulatoryDisclaimer1": "For general laboratory use.", "RegulatoryDisclaimer2": "", "StainLocalization": [ "" ], "ProductType": "Others", "DisclaimerGroup2": null, "ProductNameGlobal": "High Pure PCR Template Preparation Kit", "DisclaimerGroup1": "GPR", "ControlTissue": [ "" ], "BrandName": "High Pure PCR Template Preparation Kit", "LicenseDisclaimers": [ ], "Isotypes": "", "RegulatoryDisclaimer3": "", "ID": "3.6.8.48.1.1", "ProductNameAddition": "For isolation of nucleic acids for PCR and Southern Blotting", "Species": [ "" ], "Clone": "" }, "ProductImageDetails": { "ImagePath": "https://pim-media.roche.com/Images/Article_11796828001_im_en.png", "ImageType": "Image main" }, "Product2Taxomony": { "Product2TaxomonyReferences": [ { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeName": "High Pure", "NodeID": "06-0008" }, { "StructureSystemIdentifier": "OWP_Product_Types", "StructureSystemName": "Product Types", "NodeName": "Assays Reagents and Strips", "NodeID": "20-000-00" }, { "StructureSystemIdentifier": "OWP_Family", "StructureSystemName": "Product Families", "NodeName": "High Pure", "NodeID": "200" }, { "StructureSystemIdentifier": "OWP_Techniques", "StructureSystemName": "Techniques", "NodeName": "Sample Preparation", "NodeID": "130-00" }, { "StructureSystemIdentifier": "Lab_Type", "StructureSystemName": "Lab Types", "NodeName": "Research Lab", "NodeID": "150-00" }, { "StructureSystemIdentifier": "Product_Solutions", "StructureSystemName": "Product Solutions", "NodeName": "Molecular", "NodeID": "100" }, { "StructureSystemIdentifier": "Lab_Type", "StructureSystemName": "Lab Types", "NodeName": "LifeScience", "NodeID": "170-00" }, { "StructureSystemIdentifier": "Applications", "StructureSystemName": "Applications", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Health_Topics", "StructureSystemName": "Health Topics", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Pathogens", "StructureSystemName": "Pathogens", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Disease_Areas", "StructureSystemName": "Disease Areas", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" } ] }, "Product2Taxonomy": { "Product2TaxonomyReferences": [ { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeName": "High Pure", "NodeID": "06-0008" }, { "StructureSystemIdentifier": "OWP_Product_Types", "StructureSystemName": "Product Types", "NodeName": "Assays Reagents and Strips", "NodeID": "20-000-00" }, { "StructureSystemIdentifier": "OWP_Family", "StructureSystemName": "Product Families", "NodeName": "High Pure", "NodeID": "200" }, { "StructureSystemIdentifier": "OWP_Techniques", "StructureSystemName": "Techniques", "NodeName": "Sample Preparation", "NodeID": "130-00" }, { "StructureSystemIdentifier": "Lab_Type", "StructureSystemName": "Lab Types", "NodeName": "Research Lab", "NodeID": "150-00" }, { "StructureSystemIdentifier": "Product_Solutions", "StructureSystemName": "Product Solutions", "NodeName": "Molecular", "NodeID": "100" }, { "StructureSystemIdentifier": "Lab_Type", "StructureSystemName": "Lab Types", "NodeName": "LifeScience", "NodeID": "170-00" }, { "StructureSystemIdentifier": "Applications", "StructureSystemName": "Applications", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Health_Topics", "StructureSystemName": "Health Topics", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Pathogens", "StructureSystemName": "Pathogens", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Disease_Areas", "StructureSystemName": "Disease Areas", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" } ] }, "Product2Materials": { "P2MaterialReferences": [ { "MaterialNum": "11796828001", "MaterialDescription": "HP PCR Template Preparation Kit", "RegisteredProductName": "", "GTIN": "04038377013985", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "1 kit", "PackSizeDescPIM360": "up to 100 purifications", "MaterialAnnotation": "", "ReadyForUse": "false", "OrderInformation": "" } ] }, "Product2Products": { "Product2ProductReference": [ ] }, "ProductSpec": [ { "ProductSpecVariant": { "Chapters": [ { "Language": "en", "Value": "Store the kit at  +15 to +25°C.", "Country": "XG", "Code": "Storage Conditions (Product)", "Name": "Storage Conditions (Product)" }, { "Language": "en", "Value": "For isolation of nucleic acids for PCR and Southern Blotting", "Country": "XG", "Code": "Product Characteristics", "Name": "Product Characteristics" }, { "Language": "en", "Value": "Cells are lysed using a short incubation with Proteinase K in the presence of a chaotropic salt (guanidine-HCl). Cellular nucleic acids (NA) bind selectively to special glass fibers pre-packed in the High Pure Purification Filter Tube. Bound NA is purified in a series of rapid \"wash-and-spin\" steps to remove contaminating cellular components. A specially formulated Inhibitor Removal Buffer has been included for use with sample material treated with 100 U/ml of heparin. Low salt elution is used to release NA from the glass fiber. This simple method eliminates the need for organic solvent extractions and DNA precipitation, ideal for rapidly purifying many samples simultaneously.

  1. Blood, cells or tissue are lysed by incubation with a special Lysis Buffer and Proteinase K.
  2. Nucleic acids are bound to the glass fibers pre-packed in the High Pure Filter Tube.
  3. Bound nucleic acids are washed with a special Inhibitor Removal Buffer to get rid of PCR inhibitory contaminants.
  4. Washing of bound nucleic acids, purification from salts, proteins and other cellular impurities.
  5. Purified nucleic acids are recovered using the Elution Buffer.
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 Whole blood and cell cultureTissue
Total timeApprox. 20 minApprox. 2 h
Hands-on timeApprox. 12 minApprox. 30 min
", "Country": "XG", "Code": "Assay Time", "Name": "Assay Time" }, { "Language": "en", "Value": "This kit purifies nucleic acids from different sample materials, including whole blood, cultured cells, and tissue samples. Bacteria and yeast require a specific prelysis treatment using lysozyme or lyticase. Resulting nucleic acids are ready for use in PCR and restriction digest reactions.", "Country": "XG", "Code": "Applications", "Name": "Applications" }, { "Language": "en", "Value": "
Vial / BottleCapLabelFunction / DescriptionContent
1whiteTissue Lysis Buffer4 M urea, 200 mM Tris, 20 mM NaCl, 200 mM EDTA, pH 7.4 (+25°C)20 ml
2greenBinding Buffer6 M guanidine-HCl, 10 mM urea, 10 mM Tris-HCl, 20% Triton X-100 (v/v), pH 4.4 (+25°C)20 ml
3pinkProteinase K, recombinant PCR gradefor sample lysis and inactivation of endogenous DNaseLyophilizate
4ablackInhibitor Removal Buffer5 M guanidine-HCl, 20 mM Tris-HCl, pH 6.6 (+25°C) final concentration after addition of ethanol.33 ml, add 20 ml absolute ethanol
4blueWash Buffer20 mM NaCl, 2 mM Tris-HCl, pH 7.5 (+25°C)final concentration after addition of ethanol.20 ml, add 80 ml absolute ethanol
5colorlessElution buffer10 mM Tris-HCl, pH 8.5 (+25°C)40 ml
6High Pure Filter TubesTwo bags with 50 polypropylene tubes with two layers of glass fiber fleece, for use of up to 700 μl sample volume
7Collection TubesEight bags with 50polypropylene tubes (2 ml)
All solutions are clear and should not be used when precipitates have formed. Warm the solutions at +15 to +25°C or in a 37°C water bath until the precipitates have dissolved.
The buffers can show a slight yellow color.This will have no impact on the function of the buffer
", "Country": "XG", "Code": "Content", "Name": "Content" } ] } } ] }

High Pure PCR Template Preparation Kit

For isolation of nucleic acids for PCR and Southern Blotting

High Pure PCR Template Preparation Kit