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| Whole blood and cell culture | Tissue | |
|---|---|---|
| Total time | Approx. 20 min | Approx. 2 h |
| Hands-on time | Approx. 12 min | Approx. 30 min |
| Vial / Bottle | Cap | Label | Function / Description | Content |
|---|---|---|---|---|
| 1 | white | Tissue Lysis Buffer | 4 M urea, 200 mM Tris, 20 mM NaCl, 200 mM EDTA, pH 7.4 (+25°C) | 20 ml |
| 2 | green | Binding Buffer | 6 M guanidine-HCl, 10 mM urea, 10 mM Tris-HCl, 20% Triton X-100 (v/v), pH 4.4 (+25°C) | 20 ml |
| 3 | pink | Proteinase K, recombinant PCR grade | for sample lysis and inactivation of endogenous DNase | Lyophilizate |
| 4a | black | Inhibitor Removal Buffer | 5 M guanidine-HCl, 20 mM Tris-HCl, pH 6.6 (+25°C) final concentration after addition of ethanol. | 33 ml, add 20 ml absolute ethanol |
| 4 | blue | Wash Buffer | 20 mM NaCl, 2 mM Tris-HCl, pH 7.5 (+25°C)final concentration after addition of ethanol. | 20 ml, add 80 ml absolute ethanol |
| 5 | colorless | Elution buffer | 10 mM Tris-HCl, pH 8.5 (+25°C) | 40 ml |
| 6 | High Pure Filter Tubes | Two bags with 50 polypropylene tubes with two layers of glass fiber fleece, for use of up to 700 μl sample volume | ||
| 7 | Collection Tubes | Eight bags with 50polypropylene tubes (2 ml) |
For isolation of nucleic acids for PCR and Southern Blotting
