For general laboratory use. Others High Pure PCR Template Preparation Kit GLU High Pure PCR Template Preparation Kit 3.6.8.48.1.1 11796828001 HP PCR Template Preparation Kit High Pure PCR Template Preparation Kit 04038377013985 Reagents, kits 1 kit up to 100 isolations false How this product worksCells are lysed using a short incubation with Proteinase K in the presence of a chaotropic salt (guanidine-HCl). Cellular nucleic acids (NA) bind selectively to special glass fibers pre-packed in the High Pure Purification Filter Tube. Bound nucleic acid is purified in a series of rapid "wash-and-spin" steps to remove contaminating cellular components. A specially formulated Inhibitor Removal Buffer has been included for use with sample material treated with 100 U/ml of heparin. Low-salt elution is used to release nucleic acid from the glass fiber. This simple method eliminates the need for organic solvent extractions and DNA precipitation, ideal for rapidly purifying many samples simultaneously.Blood, cells, or tissue are lysed by incubation with a special Lysis Buffer and Proteinase K.Nucleic acids are bound to the glass fibers pre-packed in the High Pure Filter Tube.Bound nucleic acids are washed with a special Inhibitor Removal Buffer to get rid of PCR inhibitory contaminants.Washing of bound nucleic acids, purification from salts, proteins, and other cellular impurities.Purified nucleic acids are recovered using the Elution Buffer. en This High Pure PCR Template Preparation Kit isolates nucleic acids from different sample materials, including whole blood, cultured cells, and tissue samples.Bacteria and yeast require a specific prelysis treatment using lysozyme or lyticase.Resulting nucleic acids are ready for use in PCR and restriction digest reactions. en