For life science research only. Not for use in diagnostic procedures. Others High Pure RNA Isolation Kit LSR High Pure RNA Isolation Kit 3.6.8.50.1.1 11828665001 HP RNA Isolation Kit High Pure RNA Isolation Kit 04038377014593 Reagents, kits 1 kit 50 isolations false How this product worksIsolation of RNA is a prerequisite for the analysis of gene expression. Frequently, applied techniques, such as reverse transcriptase-PCR (RT-PCR), northern blotting, RNase protection, and primer extension require the use of intact, undegraded RNA from different sample materials, such as cultured cells, blood, yeast, and bacteria.Samples are lysed and homogenized in the presence of chaotropic salts, then applied to the spin filter tube. Nucleic acids bind specifically to the surface of the filter. Co-purified DNA is ultimately digested with DNase I. The bound RNA is purified from salts, proteins, digested DNA, and other impurities by washing steps, followed by an elution.Cultured cells are lysed by a special Lysis/Binding Buffer. At the same time, RNases are inactivated.Other sample materials require a specific pre-lysis treatment.Nucleic acids are bound to the glass fibers pre-packed in the High Pure Filter Tube.Residual contaminating DNA is digested by DNase I and applied directly onto the glass fiber fleece.Bound nucleic acids are washed with a special Wash Buffer to get rid of RT-PCR inhibitory contaminants.Further washing of bound nucleic acids purifies them from salts, proteins, and other cellular impurities.RNA is recovered using the Elution Buffer. en The High Pure RNA Isolation Kit is designed for the purification of total RNA from cultured cells. Other sample materials, such as blood, yeast, and bacteria require an additional specific pre-lysis treatment, which is described in the protocol section.Due to the integrated DNase digestion step, contamination of the isolated RNA with residual genomic DNA is mostly avoided.In addition, RNA is suited for other techniques, such as northern blotting, RNase protection, and primer extension.Up to 24 samples can be processed simultaneously in approximately 1 hour. Thus, the isolation procedure is less time consuming compared with alternative methods which require extraction with organic solutions, RNA precipitation, or ultracentrifugation. en