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Solution	Storage
Proteinase K	-15 to -25°C
poly(A) carrier RNA	-15 to -25°C

", "Language": "en", "Country": "XG", "Code": "Storage Conditions (Working Solution)" }, { "Name": "Storage Conditions (Product)", "Value": "Store the set at +15 to +25°C.", "Language": "en", "Country": "XG", "Code": "Storage Conditions (Product)" }, { "Name": "Assay Time", "Value": "

Total time	Approx. 20 minutes
Hands-on time	<10 minutes

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The samples are lysed by incubation with a special buffer containing Proteinase K and guanidine hydrochloride that releases nucleic acids (Cory, S. et al., 1983). A highly efficient reaction is obtained at elevated temperatures. Subsequently, the liquid is centrifuged through a glass fiber filter that is contained in the kit (Yang, R. et al., 1979). During this process, the nucleic acids are bound specifically to the surface of the glass fiber (Jakobi, R. et al., 1988; Kristensen, T. et al., 1987). Unbound substances are removed by centrifugation. The absorbed nucelic acids are washed and eluted with an aqueous solution.

1. The isolation of the analyte from serum or plasma is required. Virus lysis is accomplished by incubating the sample in a special Lysis / Binding Buffer.
2. Isolation of the nucleic acids by binding to the glass fibers.
3. Washing of bound nucleic acids, purification from salts, proteins and other cellular impurities.
4. Elution of the purified NA with Elution Buffer.

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Vial / Bottle	Cap	Label	Function / Description	Content
1	green	Lysis/Binding Buffer	Contains 6 M guanidine-HCl, 10 mM urea, 10 mM Tris-HCl, 20% Triton X-100 (v/v), pH 4.4 (+25°C)	25 ml
2	white	Poly(A)	Lyophilizate for binding of RNA	2 mg poly(A) carrier RNA
3	pink	Proteinase K	Lyophilizate for the digestion of proteins	100 mg
4a	black	Inhibitor Removal Buffer	Contains 5 M guanidine-HCl, 20 mM Tris-HCl, pH 6.6 (+25°C) (final concentration after addition of ethanol)	33 ml, add 20 ml absolute ethanol
4	blue	Wash Buffer	Contains 20 mM NaCl, 2 mM Tris-HCl, pH 7.5 (+25°C) (final concentrations after addition of ethanol)	20 ml add 80 ml absolute ethanol
5	colorless	Elution Buffer	Water PCR grade	30 ml

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