For general laboratory use. Others High Pure Viral Nucleic Acid Kit GLU High Pure Viral Nucleic Acid Kit 3.6.8.55.1.1 11858874001 HP Viral Nucleic Acid Kit High Pure Viral Nucleic Acid Kit 04038377014951 Reagents, kits 1 kit up to 100 isolations false The High Pure Viral Nucleic Acid Kit is designed to isolate viral nucleic acids from mammalian serum, plasma, or whole blood.When using whole blood, total nucleic acids are isolated, including viral nucleic acids.The isolated viral nucleic acids are applied in PCR or RT-PCR directly after elution in PCR-grade water. en How this product worksAs a pre-requisite for the analysis of viral nucleic acids by PCR or RT-PCR, the isolation of the analyte from serum, plasma, or whole blood is required.Virus lysis is accomplished by incubation of the sample in a special Lysis/Binding Buffer in the presence of Proteinase K. Subsequently, nucleic acids bind specifically to the surface of glass fibers in the presence of a chaotropic salt. The binding reaction occurs within seconds due to the disruption of the organized structure of water molecules and the interaction of nucleic acids with the glass fibers surface. Thus, adsorption to the glass fiber fleece is favored. Since the binding process is specific for nucleic acids, the bound nucleic acids are purified from salts, proteins, and other impurities by a washing step and are eluted in low-salt buffer or water.Serum, plasma, or whole blood are lysed by incubation with Binding Buffer and Proteinase K.Nucleic acids are bound to the glass fibers pre-packed in the High Pure Filter Tube.Bound nucleic acids are washed with a special Inhibitor Removal Buffer to get rid of PCR inhibitory contaminants.– Allows even the application of heparinized sample material with >100 U/mL heparin.Washing of bound nucleic acids, purification from salts, proteins, and other cellular impurities.Purified nucleic acids are recovered using the Elution Buffer. en