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When using whole blood total nucleic acids are purified including viral nucleic acids. The purified viral nucleic acids are applied in PCR or RT-PCR directly after elution in water, PCR grade.", "Language": "en", "Country": "XG", "Code": "Applications" }, { "Name": "Content", "Value": "

Vial / Bottle	Cap	Label	Contents / Function
1	green	Binding Buffer	2 × 25 ml [6 M guanidine-HCl, 10 mM Tris-HCl, 10 mM urea, 20% Triton X-100 (w/v), pH 4.4 (+25°C)]
2		Poly(A)	Lyophilizate 2 mg poly(A) carrier RNA For binding of RNA
3	pink	Proteinase K	Lyophilizate 100 mg For the digestion of proteins
4 a	black	Inhibitor Removal Buffer	33 ml, add 20 ml absolute ethanol [5 M guanidine-HCl, 20 mM Tris-HCl, pH 6.6 (+25°C) final concentration after addition of ethanol]
4 b	blue	Wash Buffer	2 × 10 ml add 40 ml absolute ethanol each [20 mM NaCl, 2 mM Tris-HCl, pH 7.5 (+25°C) final concentrations after addition of ethanol]
5	colorless	Elution Buffer	30 ml Water, PCR Grade
6		High Pure Filter Tubes	Two bags with 50 polypropylene tubes with two layers of glass fiber fleece, for use of up to 700 μl sample volume.
7		Collection Tubes	Eight bags with 50 polypropylene tubes (2 ml).

", "Language": "en", "Country": "XG", "Code": "Content" }, { "Name": "Principle", "Value": "As a pre-requisite for the analysis of viral nucleic acids by the polymerase chain reaction (PCR) or RT-PCR the isolation of the analyte from serum, plasma or whole blood is required.
Virus lysis is accomplished by incubation of the sample in a special Lysis/Binding buffer in the presence of Proteinase K. Subsequently, nucleic acids bind specifically to the surface of glass fibers in the presence of a chaotropic salt (1). The binding reaction occurs within seconds due to the disruption of the organized structure of water molecules and the interaction of nucleic acids with the glass fibers surface. Thus, adsorption to the glass fiber fleece is favored. Since the binding process is specific for nucleic acids, the bound nucleic acids are purified from salts, proteins and other impurities by a washing step and are eluted in low salt buffer or water.

1. Serum, plasma or whole blood are lysed by incubation with Binding buffer and Proteinase K.
2. Nucleic acids are bound to the glass fibers pre-packed in the High Pure Filter Tube.
3. Bound nucleic acids are washed with a special Inhibitor Removal Buffer to get rid of PCR inhibitory contaminants. It allows even the application of heparinized sample material with > 100 U/ml heparin.
4. Washing of bound nucleic acids, purification from salts, proteins and other cellular impurities.
5. Purified nucleic acids are recovered using the Elution Buffer.

", "Language": "en", "Country": "XG", "Code": "Principle" }, { "Name": "Assay Time", "Value": "

Total time	Approximately 20 min
Hands-on time	<10 min

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