{ "ProductData": { "RelatedLinks": "", "RegulatoryDisclaimer1": "For general laboratory use.", "RegulatoryDisclaimer2": "", "StainLocalization": [ "" ], "ProductType": "Others", "DisclaimerGroup2": null, "ProductNameGlobal": "High Pure Viral RNA Kit", "DisclaimerGroup1": "GPR", "ControlTissue": [ "" ], "BrandName": "High Pure Viral RNA Kit", "LicenseDisclaimers": [ ], "Isotypes": "", "RegulatoryDisclaimer3": "", "ID": "3.6.8.54.1.1", "ProductNameAddition": "For the isolation of viral RNA for RT-PCR", "Species": [ "" ], "Clone": "" }, "ProductImageDetails": { "ImagePath": "https://pim-media.roche.com/Images/Article_11858882001_im_en.png", "ImageType": "Image main" }, "Product2Taxomony": { "Product2TaxomonyReferences": [ { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeName": "High Pure", "NodeID": "06-0008" }, { "StructureSystemIdentifier": "OWP_Product_Types", "StructureSystemName": "Product Types", "NodeName": "Assays Reagents and Strips", "NodeID": "20-000-00" }, { "StructureSystemIdentifier": "OWP_Family", "StructureSystemName": "Product Families", "NodeName": "High Pure", "NodeID": "200" }, { "StructureSystemIdentifier": "OWP_Techniques", "StructureSystemName": "Techniques", "NodeName": "Sample Preparation", "NodeID": "130-00" }, { "StructureSystemIdentifier": "Lab_Type", "StructureSystemName": "Lab Types", "NodeName": "Research Lab", "NodeID": "150-00" }, { "StructureSystemIdentifier": "Health_Topics", "StructureSystemName": "Health Topics", "NodeName": "Infectious Diseases", "NodeID": "17-00-00" }, { "StructureSystemIdentifier": "Product_Solutions", "StructureSystemName": "Product Solutions", "NodeName": "Molecular", "NodeID": "100" }, { "StructureSystemIdentifier": "Lab_Type", "StructureSystemName": "Lab Types", "NodeName": "LifeScience", "NodeID": "170-00" }, { "StructureSystemIdentifier": "Applications", "StructureSystemName": "Applications", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Pathogens", "StructureSystemName": "Pathogens", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Disease_Areas", "StructureSystemName": "Disease Areas", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" } ] }, "Product2Taxonomy": { "Product2TaxonomyReferences": [ { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeName": "High Pure", "NodeID": "06-0008" }, { "StructureSystemIdentifier": "OWP_Product_Types", "StructureSystemName": "Product Types", "NodeName": "Assays Reagents and Strips", "NodeID": "20-000-00" }, { "StructureSystemIdentifier": "OWP_Family", "StructureSystemName": "Product Families", "NodeName": "High Pure", "NodeID": "200" }, { "StructureSystemIdentifier": "OWP_Techniques", "StructureSystemName": "Techniques", "NodeName": "Sample Preparation", "NodeID": "130-00" }, { "StructureSystemIdentifier": "Lab_Type", "StructureSystemName": "Lab Types", "NodeName": "Research Lab", "NodeID": "150-00" }, { "StructureSystemIdentifier": "Health_Topics", "StructureSystemName": "Health Topics", "NodeName": "Infectious Diseases", "NodeID": "17-00-00" }, { "StructureSystemIdentifier": "Product_Solutions", "StructureSystemName": "Product Solutions", "NodeName": "Molecular", "NodeID": "100" }, { "StructureSystemIdentifier": "Lab_Type", "StructureSystemName": "Lab Types", "NodeName": "LifeScience", "NodeID": "170-00" }, { "StructureSystemIdentifier": "Applications", "StructureSystemName": "Applications", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Pathogens", "StructureSystemName": "Pathogens", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Disease_Areas", "StructureSystemName": "Disease Areas", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" } ] }, "Product2Materials": { "P2MaterialReferences": [ { "MaterialNum": "11858882001", "MaterialDescription": "HP Viral RNA Kit", "RegisteredProductName": "High Pure Viral RNA Kit", "GTIN": "04038377014968", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "1 kit", "PackSizeDescPIM360": "up to 100 purifications", "MaterialAnnotation": "", "ReadyForUse": "", "OrderInformation": "" } ] }, "Product2Products": { "Product2ProductReference": [ ] }, "ProductSpec": [ { "ProductSpecVariant": { "Chapters": [ { "Language": "en", "Value": "Store the kit at  +15 to +25°C.", "Country": "XG", "Code": "Storage Conditions (Product)", "Name": "Storage Conditions (Product)" }, { "Language": "en", "Value": "As a pre-requisite for the analysis of viral RNA by the reverse transcription polymerase chain reaction (RT-PCR) the isolation of the analyte from serum or plasma is required.
The High Pure Viral RNA Kit accomplishes virus lysis by incubation of the sample in a special Binding Buffer. Subsequently nucleic acids bind specifically to the surface of glass fibers in the presence of a chaotropic salt (guanidine-HCl). The binding reaction occurs within seconds due to the disruption of the organized structure of water molecules and the interaction of nucleic acids with the glass fibers surface. Thus, adsorption to the glass fiber fleece is favored. Since the binding process is specific for nucleic acids, the bound nucleic acids are purified from salts, proteins and other impurities by a washing step and are finally eluted in low salt Elution Buffer or PCR grade water. The purified viral RNA is free of intact virus, nucleases, and all cellular components that interfere with RT-PCR and can be applied directly for RT-PCR. 50 μl eluate is sufficient for 8-14 RT-PCR reactions.
Included in the kit is a special Inhibitor Removal Buffer resulting in improved sensitivity and reproducibility of RT-PCR assays performed with nucleic acid templates isolated with this kit. Especially, the use of the Inhibitor Removal Buffer allows even the application of heparinized sample material containing 100 U/ml heparin.

The High Pure Viral RNA Kit
  • saves time, because the kit does not require extraction with organic solutions or nucleic acid precipitation and thus can prepare multiple RT-PCR templates in approximately 10 minutes
  • accommodates a wide variety of samples, because the same kit can purify viral RNA from several bodily fluids
  • minimizes RNA loss, because the kit removes contaminants without precipitation or solvent extraction
  • increases lab safety, because the kit minimizes the handling of potentially hazardous samples and does not use hazardous organic solvents

  1. Serum or plasma are lysed by incubation with Binding Buffer.
  2. Nucleic acids are bound to the glass fibers pre-packed in the High Pure Filter Tube.
  3. Bound nucleic acids are washed with a special Inhibitor Removal Buffer to get rid of RT-PCR inhibitory contaminants. It allows even the application of heparinized sample material with >100 U/ml heparin.
  4. Washing of bound nucleic acids, purification from salts, proteins and other cellular impurities.
  5. Purified nucleic acids are recovered using the Elution Buffer.
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Vial / BottleCapLabelFunction / DescriptionContent
1greenBinding BufferContains 4.5 M guanidine-HCl, 50 mM Tris-HCl, 30% Triton X-100 (w/v), pH 6.6 (+25°C).
  • 2 × 25 ml
2 Poly (A) For binding of RNA 
  • 2 mg poly(A) carrier RNA (lyophilizate)
3ablack        Inhibitor Removal Buffer Contains 5 M guanidine-HCl, 20 mM Tris-HCl, pH 6.6 (+25°C) (final concentration after addition of ethanol)
  •  33 ml, add 20 ml absolute ethanol
3blueWash Buffer20 mM NaCl, 2 mM Tris-HCl, pH 7.5 (+25°C) (final concentrations after addition of ethanol)
  • 2 × 10 ml, add 40 ml absolute ethanol to each vial
4colorlessElution BufferWater, PCR grade
  • 30 ml
5 High Pure Filter TubesFor use of up to 700 μl sample volume.
  • 2 bags with 50 polypropylene tubes with two layers of glass fiber fleece
6 Collection Tubes 
  • 8 bags with 50 polypropylene tubes (2 ml).
All solutions are clear and should not be used when precipitates have formed. Warm the solutions at +15 to +25°C or in a 37°C water bath until the precipitates have dissolved.
The buffers can show a slight yellow color. This will have no impact on the function of the buffer.
", "Country": "XG", "Code": "Content", "Name": "Content" }, { "Language": "en", "Value": "The High Pure Viral RNA Kit is designed to purify intact viral RNA from serum or plasma samples. Viral RNA is used for RT-PCR analysis directly after elution in PCR grade water.
RNA preparations obtained are suitable for RT-PCR; they are not tested for other applications.
", "Country": "XG", "Code": "Applications", "Name": "Applications" }, { "Language": "en", "Value": "
Total timeApprox. 20 min
Hands-on time<10 min
", "Country": "XG", "Code": "Assay Time", "Name": "Assay Time" }, { "Language": "en", "Value": "For the isolation of viral RNA for RT-PCR", "Country": "XG", "Code": "Product Characteristics", "Name": "Product Characteristics" }, { "Language": "en", "Value": "The High Pure Viral RNA Kit is designed to purify viral RNA for RT-PCR analysis.", "Country": "XG", "Code": "Product Description", "Name": "Product Description" } ] } } ] }

High Pure Viral RNA Kit

For the isolation of viral RNA for RT-PCR

High Pure Viral RNA Kit