For general laboratory use. Others High Pure Viral RNA Kit GLU High Pure Viral RNA Kit 3.6.8.54.1.1 11858882001 HP Viral RNA Kit High Pure Viral RNA Kit 04038377014968 Reagents, kits 1 kit 100 isolations The High Pure Viral RNA Kit is designed to purify viral RNA for RT-PCR analysis. en The High Pure Viral RNA Kit is designed to isolate intact viral RNA from serum or plasma samples. Viral RNA is used for RT-PCR analysis directly after elution in PCR-grade water.RNA preparations obtained are suitable for RT-PCR; they are not tested for other applications. en How this product worksAs a pre-requisite for the analysis of viral RNA by the reverse transcription polymerase chain reaction (RT-PCR), the isolation of the analyte from serum or plasma is required. The High Pure Viral RNA Kit accomplishes virus lysis by incubation of the sample in a special Binding Buffer. Subsequently nucleic acids bind specifically to the surface of glass fibers in the presence of a chaotropic salt (guanidine-HCl). The binding reaction occurs within seconds due to the disruption of the organized structure of water molecules and the interaction of nucleic acids with the glass fibers surface. Thus, adsorption to the glass fiber fleece is favored. Since the binding process is specific for nucleic acids, the bound nucleic acids are purified from salts, proteins, and other impurities by a washing step and are finally eluted in low-salt Elution Buffer or PCR-grade water. The purified viral RNA is free of intact virus, nucleases, and all cellular components that interfere with RT-PCR, and can be applied directly for RT-PCR. Fifty microliter eluate is sufficient for 8 to 14 RT-PCR reactions.Included in the kit is a special Inhibitor Removal Buffer that results in improved sensitivity and reproducibility of RT-PCR assays performed with nucleic acid templates isolated with this kit. The use of the Inhibitor Removal Buffer allows even the application of heparinized sample material containing 100 U/mL heparin.Serum or plasma are lysed by incubation with Binding Buffer.Nucleic acids are bound to the glass fibers pre-packed in the High Pure Filter Tube.Bound nucleic acids are washed with a special Inhibitor Removal Buffer to remove RT-PCR inhibitory contaminants.– Allows even the application of heparinized sample material with >100 U/mL heparin.Washing of bound nucleic acids, purification from salts, proteins. and other cellular impurities.Purified nucleic acids are recovered using the Elution Buffer. en