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Vial / bottle	Cap	Label	Function / description	Content
1	green	High Pure Viral RNA Kit, Binding Buffer	Contains 4.5 M guanidine-HCl, 50 mM Tris-HCl, 25% polidocanol (w/v).	2 bottles, 25 mL each
2	–	High Pure Viral RNA Kit, Poly (A)	For binding of RNA. Lyophilized	1 vial, 2 mg Poly (A) carrier RNA
3a	black	High Pure Viral RNA Kit, Inhibitor Removal Buffer	Contains 5 M guanidine-HCl, 20 mM Tris-HCl, pH 6.6 (+25°C); final concentration after addition of ethanol. See Section Working Solution for information on preparing the solution.	1 bottle, 33 mL
3	blue	High Pure Viral RNA Kit, Wash Buffer	Contains 20 mM NaCl, 2 mM Tris-HCl, pH 7.5 (+25°C); final concentrations after addition of ethanol. See Section Working Solution for information on preparing the solution.	2 bottles, 10 mL each
4	colorless	High Pure Viral RNA Kit, Elution Buffer	Water, PCR grade	1 bottle, 30 mL
5	–	High Pure Viral RNA Kit, High Pure Filter Tubes	For use of up to 700 μL sample volume.	2 bags, 50 polypropylene filter tubes with two layers of glass fiber fleece each
6	–	High Pure Viral RNA Kit, Collection Tubes	For viral RNA isolation.	8 bags, 50 polypropylene tubes, 2 mL each

", "Language": "en", "Country": "XG", "Code": "Content" }, { "Name": "Product Description", "Value": "The High Pure Viral RNA Kit is designed to purify viral RNA for RT-PCR analysis.", "Language": "en", "Country": "XG", "Code": "Product Description" }, { "Name": "Assay Time", "Value": " 

Total time	Approximately 20 minutes.
Hands-on time	<10 minutes.

", "Language": "en", "Country": "XG", "Code": "Assay Time" }, { "Name": "Principle", "Value": "As a pre-requisite for the analysis of viral RNA by the reverse transcription polymerase chain reaction (RT-PCR), the isolation of the analyte from serum or plasma is required. The High Pure Viral RNA Kit accomplishes virus lysis by incubation of the sample in a special Binding Buffer. Subsequently nucleic acids bind specifically to the surface of glass fibers in the presence of a chaotropic salt (guanidine-HCl). The binding reaction occurs within seconds due to the disruption of the organized structure of water molecules and the interaction of nucleic acids with the glass fibers surface. Thus, adsorption to the glass fiber fleece is favored. Since the binding process is specific for nucleic acids, the bound nucleic acids are purified from salts, proteins, and other impurities by a washing step and are finally eluted in low-salt Elution Buffer or PCR-grade water. The purified viral RNA is free of intact virus, nucleases, and all cellular components that interfere with RT-PCR, and can be applied directly for RT-PCR. Fifty microliter eluate is sufficient for 8 to 14 RT-PCR reactions.
Included in the kit is a special Inhibitor Removal Buffer that results in improved sensitivity and reproducibility of RT-PCR assays performed with nucleic acid templates isolated with this kit. The use of the Inhibitor Removal Buffer allows even the application of heparinized sample material containing 100 U/mL heparin.

1. Serum or plasma are lysed by incubation with Binding Buffer.
2. Nucleic acids are bound to the glass fibers pre-packed in the High Pure Filter Tube.
3. Bound nucleic acids are washed with a special Inhibitor Removal Buffer to remove RT-PCR inhibitory contaminants.
   – Allows even the application of heparinized sample material with >100 U/mL heparin.
4. Washing of bound nucleic acids, purification from salts, proteins. and other cellular impurities.
5. Purified nucleic acids are recovered using the Elution Buffer.

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