Vial / bottle | Cap | Label | Function / description | Content |
---|---|---|---|---|
1 | green | High Pure Viral RNA Kit, Binding Buffer | Contains 4.5 M guanidine-HCl, 50 mM Tris-HCl, 25% polidocanol (w/v). | 2 bottles, 25 mL each |
2 | – | High Pure Viral RNA Kit, Poly (A) |
| 1 vial, 2 mg Poly (A) carrier RNA |
3a | black | High Pure Viral RNA Kit, Inhibitor Removal Buffer | Contains 5 M guanidine-HCl, 20 mM Tris-HCl, pH 6.6 (+25°C); final concentration after addition of ethanol. See Section Working Solution for information on preparing the solution. | 1 bottle, 33 mL |
3 | blue | High Pure Viral RNA Kit, Wash Buffer | Contains 20 mM NaCl, 2 mM Tris-HCl, pH 7.5 (+25°C); final concentrations after addition of ethanol. See Section Working Solution for information on preparing the solution. | 2 bottles, 10 mL each |
4 | colorless | High Pure Viral RNA Kit, Elution Buffer | Water, PCR grade | 1 bottle, 30 mL |
5 | – | High Pure Viral RNA Kit, High Pure Filter Tubes | For use of up to 700 μL sample volume. | 2 bags, 50 polypropylene filter tubes with two layers of glass fiber fleece each |
6 | – | High Pure Viral RNA Kit, Collection Tubes | For viral RNA isolation. | 8 bags, 50 polypropylene tubes, 2 mL each |
Total time | Approximately 20 minutes. |
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Hands-on time | <10 minutes. |