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RNA preparations obtained are suitable for RT-PCR; they are not tested for other applications.
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Vial / bottleCapLabelFunction / descriptionContent
1greenHigh Pure Viral RNA Kit,
Binding Buffer
Contains 4.5 M guanidine-HCl, 50 mM Tris-HCl, 25% polidocanol (w/v).2 bottles,
25 mL each
2High Pure Viral RNA Kit,
Poly (A)
  • For binding of RNA.
  • Lyophilized
1 vial,
2 mg Poly (A) carrier RNA
3ablackHigh Pure Viral RNA Kit,
Inhibitor Removal Buffer
Contains 5 M guanidine-HCl, 20 mM Tris-HCl, pH 6.6 (+25°C); final concentration after addition of ethanol.
See Section Working Solution for information on preparing the solution.
1 bottle,
33 mL
3blueHigh Pure Viral RNA Kit,
Wash Buffer
Contains 20 mM NaCl, 2 mM Tris-HCl, pH 7.5 (+25°C); final concentrations after addition of ethanol.
See Section Working Solution for information on preparing the solution.
2 bottles,
10 mL each
4colorlessHigh Pure Viral RNA Kit,
Elution Buffer
Water, PCR grade1 bottle,
30 mL
5High Pure Viral RNA Kit,
High Pure Filter Tubes
For use of up to 700 μL sample volume.2 bags,
50 polypropylene filter tubes with two layers of glass fiber fleece each
6High Pure Viral RNA Kit,
Collection Tubes
For viral RNA isolation.8 bags,
50 polypropylene tubes, 2 mL each
All solutions are clear and should not be used when precipitates have formed. Warm the solutions at +15 to +25°C or in a +37°C water bath until the precipitates have dissolved.
The buffers can show a slight yellow color. This will have no impact on the function of the buffer.
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Total timeApproximately 20 minutes.
Hands-on time<10 minutes.
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How this product works
As a pre-requisite for the analysis of viral RNA by the reverse transcription polymerase chain reaction (RT-PCR), the isolation of the analyte from serum or plasma is required. The High Pure Viral RNA Kit accomplishes virus lysis by incubation of the sample in a special Binding Buffer. Subsequently nucleic acids bind specifically to the surface of glass fibers in the presence of a chaotropic salt (guanidine-HCl). The binding reaction occurs within seconds due to the disruption of the organized structure of water molecules and the interaction of nucleic acids with the glass fibers surface. Thus, adsorption to the glass fiber fleece is favored. Since the binding process is specific for nucleic acids, the bound nucleic acids are purified from salts, proteins, and other impurities by a washing step and are finally eluted in low-salt Elution Buffer or PCR-grade water. The purified viral RNA is free of intact virus, nucleases, and all cellular components that interfere with RT-PCR, and can be applied directly for RT-PCR. Fifty microliter eluate is sufficient for 8 to 14 RT-PCR reactions.
Included in the kit is a special Inhibitor Removal Buffer that results in improved sensitivity and reproducibility of RT-PCR assays performed with nucleic acid templates isolated with this kit. The use of the Inhibitor Removal Buffer allows even the application of heparinized sample material containing 100 U/mL heparin.
  1. Serum or plasma are lysed by incubation with Binding Buffer.
  2. Nucleic acids are bound to the glass fibers pre-packed in the High Pure Filter Tube.
  3. Bound nucleic acids are washed with a special Inhibitor Removal Buffer to remove RT-PCR inhibitory contaminants.
    – Allows even the application of heparinized sample material with >100 U/mL heparin.
  4. Washing of bound nucleic acids, purification from salts, proteins. and other cellular impurities.
  5. Purified nucleic acids are recovered using the Elution Buffer.
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    High Pure Viral RNA Kit

    GPR For general laboratory use.
    High Pure Viral RNA Kit