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The new thermal block cycler unit and block lid are automatically detected and calibrated by the LightCycler^® 480 Instrument after the instrument is turned on. No additional calibrations are necessary.


Expected Life Time

The specified lifetime of the thermal block cycler is 105,000 cycles (corresponding to approx. 4 runs with 43 cycles on 200 days over 3 years).


Temperature Homogeneity

The thermal block cycler of the LightCycler^® 480 Instrument has three different temperature sensors: one sensor is located in the center of the plate mount and is used for absolute temperature control; the other two sensors for checking temperature homogeneity are located on top of the left and right section of the block.


No Recalibration of the LightCycler^® 480 Instrument after exchange of the thermal block cycler unit

Each thermal block cycler unit has its own EEPROM*, carrying block-specific information, such as calibration data. During instrument start-up, information within the EEPROM is read by the instrument controller and transferred to the LightCycler^® 480 Software.
(*EEPROM = Electrically Erasable Programmable Read-Only Memory)


General Cleaning of the Thermal Block Cycler

Regular cleaning of the LightCycler^® 480 Instruments and accessories is not required. If necessary, clean the housing of the LightCycler^® 480 Instrument, the thermal block cycler, and the block cycler cover with a mild commercial detergent. Use 70% ethanol for disinfecting the instrument housing, the thermal block cycler, and the block cycler cover.

Cleaning of the LightCycler^® 480 block cycler unit: Pipette into all wells 20 μl (384-well block), respectively 125 μl (96 well block), 70% ethanol or isopropanol. After 15 minutes, pipette up and down several times. Remove the liquid and let the block cycler unit dry before use. Take care not to degrade the block coating during cleaning.
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1. Thermal Block Cycler Unit for 96-well PCR plates
2. Corresponding Block Lid for 96-well PCR plates
3. Storage Box for both accessories

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